EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of serum and plasma samples for determination of tricyclic antidepressants: effects of blood collection tubes and storage


Therapeutic Drug Monitoring 8(4): 478-482
Preparation of serum and plasma samples for determination of tricyclic antidepressants: effects of blood collection tubes and storage
The effects were tested of eight common types of blood collection tubes and two types of "plasma separators" on the stability of the tricyclic antidepressants amitriptyline, imipramine, clomipramine, and their monodemethylated metabolites in venous blood samples. Although EDTA-containing Venoject lavender and Vacutainer lavender tubes seemed to give the most stable plasma samples, and Venoject red the most stable serum samples, the differences were too small to have practical consequences. Vacutainer royal blue collection tubes gave significant losses of greater than 20% of some of the substances. The tubes with serum separator gel or filter proved unsuitable, since they were responsible for losses of greater than 40%. The losses were not caused by redistribution between blood cells and plasma but occurred mainly as a result of contact between the contents and the caps of the tubes. Experiments with freezing, thawing, and storage of samples showed that freshly sampled blood could be stored at room temperature for 24 h in Venoject green tubes without significant losses. Serum samples could be stored at refrigerator temperature for 4 weeks without important losses. Freezing, thawing, and storage at -20 degrees C did not influence the serum or plasma concentrations.


Accession: 006173861

PMID: 3824436



Related references

Adsorption of tricyclic antidepressants to arylic and polyester separator gels in blood collection tubes. Clinical Chemistry 42(6 PART 2): S224, 1996

Effects of storage and type of blood collection tubes on hepatitis C virus level in whole blood samples. Journal of Clinical Microbiology 39(5): 1788-1790, 2001

Comparison among plasma, serum, and whole blood ethanol concentrations: impact of storage conditions and collection tubes. Journal of Analytical Toxicology 32(7): 505-510, 2008

Validation of non-aqueous capillary electrophoresis for simultaneous determination of four tricyclic antidepressants in pharmaceutical formulations and plasma samples. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 799(1): 127-132, 2003

Optimization of solid-phase microextraction procedures for the determination of tricyclic antidepressants and anticonvulsants in plasma samples by liquid chromatography. Analytical and Bioanalytical Chemistry 386(2): 256-263, 2006

Comparative determination of tricyclic antidepressants by three different antibodies in blood serum. Analytical and Bioanalytical Chemistry 327(1): 76-77, 1987

Comparison of serum from red top and experimental serum separator gel tubes in the assay of tricyclic antidepressants. Clinical Chemistry 37(6): 994, 1991

Determination of urine tricyclic antidepressants using the Abbott Axsym serum tricyclic assay. Clinical Chemistry 43(6 PART 2): S216, 1997

Agreement in the determination of electrolytes in samples of serum and plasma. Stability of these samples kept for 2 hours at room temperature with uncovered tubes. Annales de Biologie Clinique 64(2): 157-161, 2006

Blood collection by evacuated tubes: the effects of the order of filling tubes on the serum values of potassium and lactate dehydrogenase. Annales de Biologie Clinique 47(8): 507-507, 1989