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Preparation of simple biological dosimeter and its use in the evaluation of the sterilization effect of low energy electron beams

Report of National Food Research Institute (46): 9-14
Preparation of simple biological dosimeter and its use in the evaluation of the sterilization effect of low energy electron beams
To investigate the sterilization effect [for food processing] of low energy electron beams, a simple biological dosimeter (BD) was prepared. Spores of Bacillus pumilus E601 were used as microbial indicator. One ml of spore suspension, to which 1 ml of 0.1% Tween-80 solution and 8 ml of ethanol was added, was spread onto 30 .times. 20 cm aluminum foil (10 .mu.). The BD was applied in placing about 1 .times. 107 spores on each sheet of aluminum fold measuring in surface area. D10 values for electron beams of 3 radiators, i.e., Electrocurtain CB-200 (150-200 kV), self-shield type EBP-500-30 (300-550 kV) and Van de Graaff (1.0-2.5 MV) were determined. D10 value for 60Co-gamma ray was also determined as a reference. D10 values tended to decrease with the decrease in the radiation energy of the electron beams. The 150-550 keV electron beams were as effective in surface sterilization as higher energy (1.0-2.5 MeV) beams.

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Accession: 006173863

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