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Preparation of simple biological dosimeter and its use in the evaluation of the sterilization effect of low energy electron beams



Preparation of simple biological dosimeter and its use in the evaluation of the sterilization effect of low energy electron beams



Report of National Food Research Institute (46): 9-14



To investigate the sterilization effect [for food processing] of low energy electron beams, a simple biological dosimeter (BD) was prepared. Spores of Bacillus pumilus E601 were used as microbial indicator. One ml of spore suspension, to which 1 ml of 0.1% Tween-80 solution and 8 ml of ethanol was added, was spread onto 30 .times. 20 cm aluminum foil (10 .mu.). The BD was applied in placing about 1 .times. 107 spores on each sheet of aluminum fold measuring in surface area. D10 values for electron beams of 3 radiators, i.e., Electrocurtain CB-200 (150-200 kV), self-shield type EBP-500-30 (300-550 kV) and Van de Graaff (1.0-2.5 MV) were determined. D10 value for 60Co-gamma ray was also determined as a reference. D10 values tended to decrease with the decrease in the radiation energy of the electron beams. The 150-550 keV electron beams were as effective in surface sterilization as higher energy (1.0-2.5 MeV) beams.

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Accession: 006173863

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