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Preparation of sodium potassium atpase ec 3.6.1.3 containing liposomes with predictable transport properties by a procedure relating the sodium potassium transport capacity to the atpase activity


Journal of Biochemical and Biophysical Methods 5(6): 299-306
Preparation of sodium potassium atpase ec 3.6.1.3 containing liposomes with predictable transport properties by a procedure relating the sodium potassium transport capacity to the atpase activity
A microprocedure for the preparation of Na,K-ATPase-containing liposomes with a minimal starting material (200 .mu.g) of purified Na,K-ATPase is presented. Phosphatidylcholine is added gradually to cholate-solubilized Na,K-ATPase of various concentrations and the lipid-induced decrease in enzyme activity is monitored. After removal of the detergent by dialysis, the transport parameters of the resulting Na,K-ATPase-liposomes are established by a microassay. By relating the transport properties to the Na,K-ATPase activity present before dialysis, a procedure is developed which allows to prepare standardized Na,K-ATPase-liposomes with predictable transport properties.


Accession: 006173879



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