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Preparation of specific anti sera against adenoviruses by affinity bead immunization


, : Preparation of specific anti sera against adenoviruses by affinity bead immunization. Journal of Immunological Methods 26(2): 141-149

Certain adenovirus types can be replicated only to low titer in tissue cultures. Adenovirus strains associated with infantile gastroenteritis, cannot be replicated in vitro. A method which allows preparation of specific antisera was evaluated. The procedure involves coupling of group-specific antibodies against adenovirus capsid subunits to CNBr[cyanogen bromide]-activated Sepharose 4B, reaction of crude virus suspensions with immobilized adenovirus-specific Ig[immunoglobulin]G, elimination of contaminating material by extensive washing using a wide pH range and immunization [of rabbits] with adenovirus immunogens immobilized on the beads. Efficient immunization was obtained with immunogen doses of 50 ng and 50 .mu.g. The immunization procedure called affinity bead immunization (ABI) could have a wide applicability in cases where the relevant immunogen constitutes a minor fraction of a crude preparation.

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