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Preparation of specific anti sera against adenoviruses by affinity bead immunization

Journal of Immunological Methods 26(2): 141-149

Preparation of specific anti sera against adenoviruses by affinity bead immunization

Certain adenovirus types can be replicated only to low titer in tissue cultures. Adenovirus strains associated with infantile gastroenteritis, cannot be replicated in vitro. A method which allows preparation of specific antisera was evaluated. The procedure involves coupling of group-specific antibodies against adenovirus capsid subunits to CNBr[cyanogen bromide]-activated Sepharose 4B, reaction of crude virus suspensions with immobilized adenovirus-specific Ig[immunoglobulin]G, elimination of contaminating material by extensive washing using a wide pH range and immunization [of rabbits] with adenovirus immunogens immobilized on the beads. Efficient immunization was obtained with immunogen doses of 50 ng and 50 .mu.g. The immunization procedure called affinity bead immunization (ABI) could have a wide applicability in cases where the relevant immunogen constitutes a minor fraction of a crude preparation.

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Accession: 006173913

PMID: 86586

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