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Preparation of specific anti sera to digoxin using hapten carbon 17 and carbon 12 bovine serum albumin conjugates


Chemical & Pharmaceutical Bulletin (Tokyo) 32(6): 2301-2306
Preparation of specific anti sera to digoxin using hapten carbon 17 and carbon 12 bovine serum albumin conjugates
The preparation and antigenic properties of etianic acid-[C-17]- and digoxin-[C-12]-bovine serum albumin (BSA) conjugates are described. The antisera raised against the conjugate having an [O-[N-(carboxymethyl)carbamoyl]methyl]oxime bridge at C-12 in rabbits were highly specific to digoxin, exhibiting no significant cross-reactions with digoxigenin, or its bis- and monodigitoxosides. The specificity of antisera elicited is discussed in relation to the coupled position of the hapten with BSA. [Cardiac glycosides obtained from various plant sources, notably digoxin from digitalis leaf, are widely used for the treatment of congestive heart failure and of various disturbances of cardiac rhythm. The ability to determine serum digoxin concentrations by radioimmunoassay has proved to be of considerable value in controlling the treatment of patients maintained on this cardiac glycoside and in the diagnosis of digoxin intoxication.].


Accession: 006173916



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