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Preparation of specific antibodies to catecholamines and l dopa part 1 preparation of the conjugates

, : Preparation of specific antibodies to catecholamines and l dopa part 1 preparation of the conjugates. Chemical and Pharmaceutical Bulletin (Tokyo) 25(8): 1904-1910

A method for preparation of antigens of catecholamines and L-3,4-dihydroxyphenylalanine as haptens was developed. These haptens were conjugated with carrier proteins by the Mannich reaction after N-maleylation of the haptens for protecting the amino groups of the side chain, then the maleyl groups were removed by acid hydrolysis under mild conditions. By immunization of rabbits with these conjugates, antibodies were produced. In a cross-reactivity test by Ouchterlony's method the antibodies to each one of the conjugates selectively reacted with the conjugate of that hapten even if the carrier protein was bovine serum albumin or rabbit serum albumin. The present method for preparation of the conjugates is a desirable one.

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