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Preparation of specific antigens of the bovine leukosis virus blv

, : Preparation of specific antigens of the bovine leukosis virus blv. Polskie Archiwum Weterynaryjne 24(3): 327-338

The authors presented two methods of producing BLV antigens which had been proved and are utilized now at the Veterinary Institute in Pulawy. One of them is based on cultivation of blood leukocytes from leukotic cattle and on extraction of antigenic material with ethyl ether. The second method consists in obtaining antigenic proteins from a monolayer FLK-BLV culture by means of ammonium sulphate precipitation. The preparations obtained according to the above techniques showed specific serological properties and correspond with foreign standards.

Accession: 006173928

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Related references

Grundboeck, M.; Grundboeck Jusko, J.; Wilczynska Ciemiega, K.; Rutka, J., 1986: Preparation of specific antigens of bovine leukosis virus. Suspensions of cultured granulocytes from leukotic cattle were treated with ethanol. A polypeptide 'p-24' antigen was prepared from this extract. An alternative technique was to culture the FLK-BLV infected cell line (of American origin)...

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