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Preparation of specific antisera to 3 beta hydroxy 5 cholenoic acid by immunization with conjugates attached to protein through the carbon 3 position


, : Preparation of specific antisera to 3 beta hydroxy 5 cholenoic acid by immunization with conjugates attached to protein through the carbon 3 position. Chemical & Pharmaceutical Bulletin (Tokyo) 33(2): 831-836

3.beta.-Hydroxy-5-cholenoic acid-bovine serum albumin (BSA) conjugates were prepared by coupling of the haptens with the carrier protein through a 3-hemisuccinoyl or 3-hemiglutaroyl bridge. The antisera elicited by these new immunogens showed high affinity constants (Ka = 4.88 .times. 108 and 8.75 .times. 108/M) and excellent specificity to 3.beta.-hydroxy-5-cholenoic acid, exhibiting little or no cross-reactivities with cholesterol (0.001%) and related compounds. Though the antiserum obtained with the hemisuccinate-BSA conjugate was somewhat more specific than the other, they showed substantial the same reactivity, and should be useful for direct radioimmunoassay of 3.beta.-hydroxy-5-cholenoic acid in biological fluids without any chromatographic separation step prior to assay. [Oxidation of the cholesterol side chain is one of the more significant transformations in bile acid biosynthesis.].

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Kowalska, M.; Bichonski, A., 2000: Brewing quality and yield of spring barley lines in the course of 24 years of breeding in Poland. The malting quality of Polish cultivars of spring barley was evaluated over the period 1975-98. Grain came from preliminary trials at 3 locations each year. Malting quality was evaluated on the basis of 1000-grain weight, grain filling, protein co...

Rogers P.F.; Head G.A.; Lungershausen Y.K.; Howe P.R.C., 1991: Effects of depleting central and peripheral adrenaline stores on blood pressure in stroke prone spontaneously hypertensive rats. The potential role of adrenaline, both circulating and in the central nervous system, in the maintenance of high blood pressure was examined in stroke-prone spontaneously hypertensive rats (SHRSP). .alpha.-Monofluoromethyldopa, a long-lasting inhi...

Guyodo Yohan; Acton Gary D.; Brachfeld Stefanie, 1998: Paleointensity results from ODP Leg 178 Site 1101; exploring the geomagnetic field at high latitudes, Southern Hemisphere. Eos, Transactions, American Geophysical Union: 5, Suppl., Pages 235. 1998.

Bannister, J., 1985: Sustainable harvesting of whale populations. 1. Theory and background. Unknown

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Mannheim G.W., 1970: Meeting of the diabetes convention paris france may 21 23 1970. Medizinische Welt: 86-1587

Eysel U.; Peichil L.; Neubacher U., 1983: Regenerative capacity of lesioned retinal axons in kittens and in adult cats. Neuroscience Letters (SUPPL 14): S107

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Portchinsky, J., 1899: Die Bremsen (Tabanidae) und die einfachste Methode dieselben auszurotten. Unknown

Kohler H.; Muller S.; Bona C., 1985: Internal antigen and immune network. Proceedings of the Society for Experimental Biology & Medicine 178(2): 189-195