EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of specifically labeled carbon 14 labeled lignin and carbon 14 labeled cellulose ligno celluloses and their decomposition by the micro flora of soil


Applied and Environmental Microbiology 33(6): 1247-1251
Preparation of specifically labeled carbon 14 labeled lignin and carbon 14 labeled cellulose ligno celluloses and their decomposition by the micro flora of soil
Microbial decomposition of lignocellulose in soil was studied using radioisotope techniques. Natural lignocelluloses containing 14C in either their lignin or cellulose (glucan) components were prepared by feeding plants L-[U-14C]phenylalanine or D-[U-14C]glucose, respectively, through their cut stems. Detailed chemical and chromatographic characterization of labeled lignocelluloses from 3 hardwood and 3 softwood species showed that those labeled by the [14C]glucose incorporation method contained specifically labeled cellulosic components, whereas those labeled by the [14C]phenylalanine incorporation method contained specifically labeled lignin components. Microbial degradation of these differentially labeled lignocelluloses was followed by monitoring 14CO2 evolution from selected soil samples incubated with known amounts of radiolabeled lignocelluloses. The lignin components of the 6 woods were shown to be decomposed in soil 4 to 10 times more slowly than their cellulosic components. These rates of mineralization were comparable to the generalized patterns previously reported in the literature. The present technique, however, was thought to be simpler, more sensitive, and less prone to interference than methods previously available.


Accession: 006173938



Related references

Preparation characterization and microbial degradation of specifically radio labeled carbon 14 labeled ligno celluloses from marine and fresh water macrophytes. Applied & Environmental Microbiology 47(2): 381-389, 1984

Isolation of ligno cellulose decomposing actinomycetes and degradation of specifically carbon 14 labeled ligno celluloses by 6 selected streptomyces strains. Canadian Journal of Microbiology 25(11): 1270-1276, 1979

Biological delignification of carbon 14 labeled ligno celluloses by basidiomycetes degradation and solubilization of the lignin and cellulose components. Mycologia 74(6): 943-951, 1982

Degradation of carbon 14 labeled lignin labeled wheat ligno cellulose by white rot fungi. Journal of General Microbiology 130(5): 1023-1030, 1984

Preparation of specifically labeled 14C-(lignin)- and 14C-(cellulose)-lignocelluloses and their decomposition by the microflora of soil. Applied and Environmental Microbiology 33(6): 1247-1251, 1977

Preparation of specifically labeled C-(lignin)- and C-(cellulose)-lignocelluloses and their decomposition by the microflora of soil. Applied and Environmental Microbiology 33(6): 1247-1251, 1977

Observations of carbon 14 labeled carbon di oxide breath excretion patterns using an orally administered preparation of carbon 14 labeled cellulose. British Journal of Radiology 54(644): 706, 1981

Determination of extractable and nonextractable radioactivity from small field plots 45 and 95 weeks after treatment with carbon 14 labeled dicamba carbon 14 labeled 2 4 d carbon 14 labeled triallate and carbon 14 labeled trifluralin. Journal of Agricultural & Food Chemistry 32(3): 588-593, 1984

The possibility of using alkaloids as respiration substrate catabolism of carbon 14 labeled anabasine carbon 14 labeled lupinine carbon 14 labeled aphyllidine and carbon 14 labeled aphylline under short term exposure. Izvestiya Akademii Nauk SSSR Seriya Biologicheskaya (3): 356-361, 1980

Utilization of alkaloids as respiration substrate catabolism of carbon 14 labeled anabasine carbon 14 labeled lupinine carbon 14 labeled aphyllidine and carbon 14 labeled aphylline on prolonged exposure days. Biology Bulletin of the Academy of Sciences of the USSR 8(1): 65-69, 1981