+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of specifically labeled carbon 14 labeled lignin and carbon 14 labeled cellulose ligno celluloses and their decomposition by the micro flora of soil

, : Preparation of specifically labeled carbon 14 labeled lignin and carbon 14 labeled cellulose ligno celluloses and their decomposition by the micro flora of soil. Applied and Environmental Microbiology 33(6): 1247-1251

Microbial decomposition of lignocellulose in soil was studied using radioisotope techniques. Natural lignocelluloses containing 14C in either their lignin or cellulose (glucan) components were prepared by feeding plants L-[U-14C]phenylalanine or D-[U-14C]glucose, respectively, through their cut stems. Detailed chemical and chromatographic characterization of labeled lignocelluloses from 3 hardwood and 3 softwood species showed that those labeled by the [14C]glucose incorporation method contained specifically labeled cellulosic components, whereas those labeled by the [14C]phenylalanine incorporation method contained specifically labeled lignin components. Microbial degradation of these differentially labeled lignocelluloses was followed by monitoring 14CO2 evolution from selected soil samples incubated with known amounts of radiolabeled lignocelluloses. The lignin components of the 6 woods were shown to be decomposed in soil 4 to 10 times more slowly than their cellulosic components. These rates of mineralization were comparable to the generalized patterns previously reported in the literature. The present technique, however, was thought to be simpler, more sensitive, and less prone to interference than methods previously available.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006173938

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Vang, R.; Vinh, T.N.; Burks, R.Tucker.; Barner, R.; Kurman, R.J.; Ronnett, B.M., 2005: Pseudoinfiltrative tubal metaplasia of the endocervix: a potential form of in utero diethylstilbestrol exposure-related adenosis simulating minimal deviation adenocarcinoma. We report three cases of unusual tubal-type endocervical glandular proliferations Simulating minimal deviation adenocarcinoma in women with a history of in utero diethylstilbestrol (DES) exposure. The lesions were characterized by haphazard gland...

Belfort, R.; Marbeck, P.; Hsu, C.C.; Freitas, D., 2000: Epidemiological study of 134 subjects with allergic conjunctivitis. At times it is difficult to distinguish among the subtypes of chronic allergic conjunctivitis. A prospective study with 134 patients was carried out. Patients were diagnosed with vernal keratoconjunctivitis (VKC), atopic keratoconjunctivitis (AKC)...

Sun, H.; Jiang, F.; Chen, L.; Zheng, J.; Wu, Y.; Liu, M., 2013: Determination of three phthalate esters in environmental samples by coal cinder extraction and cyclodextrin modified micellar electrokinetic chromatography. A new micellar electrokinetic chromatography (MEKC) method using beta-cyclodextrin (ß-CD) as the electrophoresis additive has been developed for the simultaneous determination of dimethyl phthalate (DMP), diethyl phthalate (DEP) and di(2-ethylhex...

Yi-Nian; Zhang-Chun; Wang-Yun; Liu-Shao-Jun; Liu-Yun, 2006: The early embryonic development of triploid crucain carp coming from allotetraploid hybrids (male) x goldfish(female). The early embryonic development of triploid crucian carp which was generated by mating the males of the allotetraploid hybrids of red crucian carp(Carassius auratus red var .) (male) x common carp (Cyprinus carpio L.) (female) with the females of...

Martens, E.C.; Roth, R.; Heuser, J.E.; Gordon, J.I., 2009: Coordinate regulation of glycan degradation and polysaccharide capsule biosynthesis by a prominent human gut symbiont. Bacteria in the distal human gut have evolved diverse abilities to metabolize complex glycans, including the capacity to degrade these compounds as nutrients and to assemble their component sugars into new polymers such as extracellular capsules....

Ulstrup, K.E.; Van Oppen, M.J.H., 2003: Geographic and habitat partitioning of genetically distinct zooxanthellae (Symbiodinium) in Acropora corals on the Great Barrier Reef. Intra- and intercolony diversity and distribution of zooxanthellae in acroporid corals is largely uncharted. In this study, two molecular methods were applied to determine the distribution of zooxanthellae in the branching corals Acropora tenuis a...

Deforche, K.; Camacho, R.J.; Grossman, Z.; Soares, M.A.; Van Laethem, K.; Katzenstein, D.A.; Harrigan, P.Richard.; Kantor, R.; Shafer, R.; Vandamme, A-Mieke.; Kantor, R.; Katzenstein, D.A.; Shafer, R.W.; Camacho, R.J.; Carvalho, A.Patricia.; Wynhoven, B.; Harrigan, P.Richard.; Cane, P.; Clarke, J.; Weber, J.; Sirivichayakul, S.; Phanuphak, P.; Soares, M.A.; Tanuri, A.; Snoeck, J.; Vandamme, A-Mieke.; Morris, L.; Rudich, H.; Grossman, Z.; Schapiro, J.M.; Rodrigues, R.; Brigido, L.F.; Holguin, A.;, 2008: Bayesian network analyses of resistance pathways against efavirenz and nevirapine. To clarify the role of novel mutations selected by treatment with efavirenz or nevirapine, and investigate the influence of HIV-1 subtype on nonnucleoside reverse transcriptase inhibitor (nNRTI) resistance pathways. By finding direct dependencies...

Rost, F., 1992: Zum Neststandort der Beutelmeise (Remiz pendulinus). Mitteilungen des Vereins Saechsischer Ornithologen, 72: 82-92

Yamashita, S.; Uchida, Y.; Kojima, S.; Sakaguchi, H.; Kimura, E.; Maeda, Y.; Uchino, M., 2012: Heatstroke in patients with Parkinson's disease. We present two Parkinson s disease (PD) patients, who experienced heatstroke. Both patients manifested central nervous system dysfunction with elevated core temperature. Despite adequate lowering of the body temperature, multiorgan-dysfunction syn...

Harel, Y.; Overpeck, M.D.; Jones, D.H.; Scheidt, P.C.; Bijur, P.E.; Trumble, A.C.; Hendershot, G.E., 1995: The quality of proxy-respondent data in NCHS surveys. American Journal of Public Health 85(4): 591-592