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Preparation of spheroplasts from citrobacter freundii cells


, : Preparation of spheroplasts from citrobacter freundii cells. Prikladnaya Biokhimiya i Mikrobiologiya 22(4): 492-499

The effect of various factors (age of the culture, pH, temperature, concentrations of EDTA, sucrose and lysosyme) on lysis of the Citrobacter freundii cells by lysozyme in the presence of EDTA was being studied. The spectra of the reaction mixture turbidity showed that the cells in Tris-HCl buffer are completely disrupted, whereas in the presence of 1.0 M sucrose formed mainly spheroplasts. A method, based on preparation of spheroplasts, was applied to localize tyrosine phenollyase in the cells of Citrobacter freundii.

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Henderson, John R., 2001: A pre- and post-MARPOL Annex V summary of Hawaiian monk seal entanglements and marine debris accumulation in the northwestern Hawaiian Islands, 1982-1998. Entanglements of Hawaiian monk seals, Monachus schauinslandi, were documented in the northwestern Hawaiian Islands (NWHI) from 1982 to 1998, and debris which presented a threat of entanglement was inventoried and removed from 1987 to 1996. A total...

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V. Lukashevich; A. Schweizer; Q. Shao; P.-H. Groop; W. Kothny, 2011: Safety and efficacy of vildagliptin versus placebo in patients with type 2 diabetes and moderate or severe renal impairment A prospective 24-week randomized placebo-controlled trial. Assess safety/tolerability and efficacy of the DPP-4 inhibitor vildagliptin in 515 patients with type 2 diabetes (T2DM) and moderate or severe renal impairment (RI). Double-blind, randomized, parallel-group, placebo-controlled, 24-week clinical tr...

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Borriello, S. P., 1998: Pathogenesis of Clostridium difficile infection. Clostridium difficile produces two major toxins referred to as toxins A and B. These are thought to be primarily responsible for the virulence of the bacterium and the major contributors to the pathogenesis of antibiotic-associated gastrointestina...

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Jensch Alfred, 1965: Geophysical measurements in the steinheim basin. Neues Jahrbuch fuer Geologie und Palaeontologie Monatshefte

Kitamura, T.; Ito, T.; Ohzeki, K., 1978: Studies on the absorption of chloride by Burley tobacco. 3. Effect of heaping the farm animal wastes in the open on the exclusion of chloride and its effects on yield, quality, chemical constituents and the smoking quality of Burley tobacco. In FYM heaped in the open Cl content was reduced to 22% in 3 months and 10% in 10 months, K content was reduced and P and Ca contents were not affected. Addition of water decreased the Cl content to 10-20% regardless of storage period. In tobacco...

Meyn, A.; Weiske, G., 1943: The fowl as research animal for para-tuberculosis. Fowls are suitable for testing for paratuberculosis. The infectious material is injd., mixed with infusorial earth, trypan blue, KI and physiol. salt soln.