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Preparation of spheroplasts of schizosaccharomyces pombe


Journal of General Microbiology 128(3): 651-654
Preparation of spheroplasts of schizosaccharomyces pombe
A method is described for the conversion of S. pombe cells to spheroplasts using Novozym 234, a commercially available enzyme preparation. Exponentially growing and stationary phase cells can be quantitatively converted to spheroplasts on a large scale. A means of disrupting these spheroplasts is described which gives an efficient release of organelles. The resulting homogenate is suitable for subcellular fractionation. The spheroplasts also can be regenerated to give dividing cells, providing an approach to genetic manipulation in S. pombe.

Accession: 006173944

DOI: 10.1099/00221287-128-3-651

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