+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of spheroplasts of schizosaccharomyces pombe

Journal of General Microbiology 128(3): 651-654
Preparation of spheroplasts of schizosaccharomyces pombe
A method is described for the conversion of S. pombe cells to spheroplasts using Novozym 234, a commercially available enzyme preparation. Exponentially growing and stationary phase cells can be quantitatively converted to spheroplasts on a large scale. A means of disrupting these spheroplasts is described which gives an efficient release of organelles. The resulting homogenate is suitable for subcellular fractionation. The spheroplasts also can be regenerated to give dividing cells, providing an approach to genetic manipulation in S. pombe.

Accession: 006173944

DOI: 10.1099/00221287-128-3-651

Download PDF Full Text: Preparation of spheroplasts of schizosaccharomyces pombe

Related references

Preparation and regeneration of protoplasts and spheroplasts for fusion and transformation of schizosaccharomyces pombe. Current Microbiology 12(3): 169-174, 1985

A method for the preparation and storage of frozen, competent Schizosaccharomyces pombe spheroplasts. Biotechniques 15(2): 238-238, 1993

Combination of 2 deoxy glucose and snail gut enzyme treatments for preparing spheroplasts of schizosaccharomyces pombe. Journal of General Microbiology 75(1): 227-229, 1973

Fractionation by differential and zonal centrifugation of spheroplasts prepared from a glucose-repressed fission yeast Schizosaccharomyces pombe 972h-. Journal of General Microbiology 93(2): 241-250, 1976

Fractionation by differential and zonal centrifugation of spheroplasts prepared from a glucose repressed fission yeast schizosaccharomyces pombe 972 h minus. Journal of General Microbiology 93(2): 242-251, 1976

Nonsense suppression in Schizosaccharomyces pombe: the Schizosaccharomyces pombe Sup3-e tRNA Ser UGA gene is active in Saccharomyces cerevisiae. MGG molecular and general genetics88(2): 219-224, 1982

Nonsense suppression in schizosaccharomyces pombe the schizosaccharomyces pombe sup 3 e seryl transfer rna u g a gene is active in saccharomyces cerevisiae. Molecular & General Genetics 188(2): 219-224, 1982

N-acetylglucosamine-1-phosphate transferase of Chinese hamster ovary cells is stably expressed in Schizosaccharomyces pombe and can functionally replace the Schizosaccharomyces pombe gene. Glycobiology 2(5): 482, 1992

Switching genes in schizosaccharomyces pombe and dna rearrangements in the mating type region of schizosaccharomyces pombe. Klar, A And J N Strathern (Ed ) Current Communications in Molecular Biology: Mechanisms Of Yeast Recombination; Meeting, Cold Spring Harbor, N Y , Usa Ix+193p Cold Spring Harbor Laboratory: Cold Spring Harbor, N Y , Usa Illus Paper 35-40, 1986

The mitochondrial genome of the fission yeast Schizosaccharomyces pombe: highly homologous introns are inserted at the same position of the otherwise less conserved coxl genes in Schizosaccharomyces pombe and Aspergillus nidulans. EMBO journal European Molecular Biology Organization 3(9): 2129-2136, 1984