+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of stable and solvent free model membranes

Biochemical & Biophysical Research Communications 87(3): 789-794

Preparation of stable and solvent free model membranes

A method for the preparation of solvent-free phospholipid-impregnated filters is described. Polycarbonate filters of 13 mm diameter, 5 .mu.m thickness and 0.1 .mu.m pore size were employed, and 20-48 nmol phospholipid/filter was incorporated. Passive permeation of polar substances across the filter was determined by a flow-dialysis procedure. The presence of phospholipid led to a decrease in passive permeability by 96-99%. Due to their size and stability, and a partially preferential lipid orientation, these phospholipid-impregnated filters may serve as an alternative type of model membrane.

(PDF 0-2 workdays service: $29.90)

Accession: 006173952

PMID: 454426

Related references

Preparation of solvent-free, pore-spanning lipid bilayers: modeling the low tension of plasma membranes. Langmuir 27(12): 7672-7680, 2011

One-particle-thick, solvent-free, coarse-grained model for biological and biomimetic fluid membranes. Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics 82(1 Pt 1): 011905-011905, 2011

Solvent-free or low-solvent large-scale preparation of chloropyrimidine and analogues. Journal of Organic Chemistry 76(10): 4149-4153, 2011

Solvent-free model for self-assembling fluid bilayer membranes: stabilization of the fluid phase based on broad attractive tail potentials. Journal of Chemical Physics 123(22): 224710-224710, 2005

Reintroducing explicit solvent to a solvent-free coarse-grained model. Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics 84(2 Pt 2): 025701-025701, 2012

Preparation and evaluation of free films: influence of method of preparation and solvent composition upon the permeability. Pharmaceutica Acta Helvetiae 52(3): 47-50, 1977

ES/IS: Estimation of conformational free energy by combining dynamics simulations with explicit solvent with an implicit solvent continuum model. Biophysical Chemistry 78(1-2): 195-205, April 5, 1999

Preparation and evaluation of free films: influence of the method of preparation and of solvent composition upon the macroscopic and microscopic properties of the film. Journal de Pharmacie de Belgique 32(6): 569-577, 1977

Orally administered solvent-free pharmaceutical preparation with delayed active-substance release, and a method of preparing the preparation. Official Gazette of the United States Patent & Trademark Office Patents 1178(4): 2348, Sept 26, 1995

Synthesis of solvent stable polymeric membranes via UV depth-curing. Chemical Communications 49(98): 11494-6, 2013