EurekaMag.com logo
+ Translate

Preparation of stable and solvent free model membranes


, : Preparation of stable and solvent free model membranes. Biochemical & Biophysical Research Communications 87(3): 789-794

A method for the preparation of solvent-free phospholipid-impregnated filters is described. Polycarbonate filters of 13 mm diameter, 5 .mu.m thickness and 0.1 .mu.m pore size were employed, and 20-48 nmol phospholipid/filter was incorporated. Passive permeation of polar substances across the filter was determined by a flow-dialysis procedure. The presence of phospholipid led to a decrease in passive permeability by 96-99%. Due to their size and stability, and a partially preferential lipid orientation, these phospholipid-impregnated filters may serve as an alternative type of model membrane.

(PDF 0-2 workdays service)

Accession: 006173952

PMID: 454426

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Kocun, M.; Lazzara, T.D.; Steinem, C.; Janshoff, A., 2011: Preparation of solvent-free, pore-spanning lipid bilayers: modeling the low tension of plasma membranes. Plasma membrane tension, produced by the underlying cytoskeleton, governs many dynamic processes such as fusion, blebbing, exo- and endocytosis, cell migration, and adhesion. Here, a new protocol is introduced to model this intricate and often ove...

Yuan, H.; Huang, C.; Li, J.; Lykotrafitis, G.; Zhang, S., 2011: One-particle-thick, solvent-free, coarse-grained model for biological and biomimetic fluid membranes. Biological membranes are involved in numerous intriguing biophysical and biological cellular phenomena of different length scales, ranging from nanoscale raft formation, vesiculation, to microscale shape transformations. With extended length and t...

Sun, Z.; Wang, H.; Wen, K.; Li, Y.; Fan, E., 2011: Solvent-free or low-solvent large-scale preparation of chloropyrimidine and analogues. Chloropyrimidine or other N-containing aromatic heterocyclic analogues can be efficiently prepared from the corresponding hydroxylated precursors under solvent-free or low-solvent conditions with equimolar or less chlorinating reagents. This high-...

Cooke, I.R.; Deserno, M., 2005: Solvent-free model for self-assembling fluid bilayer membranes: stabilization of the fluid phase based on broad attractive tail potentials. We present a simple and highly adaptable method for simulating coarse-grained lipid membranes without explicit solvent. Lipids are represented by one head bead and two tail beads, with the interaction between tails being of key importance in stabi...

Kim, S.Ha.; Lamm, M.H., 2012: Reintroducing explicit solvent to a solvent-free coarse-grained model. A unique coarse-grained modeling scheme that combines a systematic, solvent-free multiscale coarse-graining algorithm for a complex macromolecule with an existing coarse-grained solvent model is proposed. We show that this procedure efficiently an...

Spitael, J.; Kinget, R., 1977: Preparation and evaluation of free films: influence of method of preparation and solvent composition upon the permeability. Pharmaceutica Acta Helvetiae 52(3): 47-50

Vorobjev, Yury N.; Hermans, Jan, 1999: ES/IS: Estimation of conformational free energy by combining dynamics simulations with explicit solvent with an implicit solvent continuum model. This paper reviews a recently developed method for calculating the total conformational free energy of a solute macromolecule in water solvent. The method consists of a relatively short simulation by molecular dynamics with explicit solvent molecu...

Vorobjev, Y.N.; Hermans, J., 1999: ES/IS: estimation of conformational free energy by combining dynamics simulations with explicit solvent with an implicit solvent continuum model. This paper reviews a recently developed method for calculating the total conformational free energy of a solute macromolecule in water solvent. The method consists of a relatively short simulation by molecular dynamics with explicit solvent molecu...

Spitael, J.; Kinget, R., 1977: Preparation and evaluation of free films: influence of the method of preparation and of solvent composition upon the macroscopic and microscopic properties of the film. Journal de Pharmacie de Belgique 32(6): 569-577

Muench, U.; Mika, H J.; Emschermann, B.; Schmidt, R.; Sczepanik, B., 1995: Orally administered solvent-free pharmaceutical preparation with delayed active-substance release, and a method of preparing the preparation. Official Gazette of the United States Patent & Trademark Office Patents 1178(4): 2348, Sept 26