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Preparation of stable and solvent free model membranes

, : Preparation of stable and solvent free model membranes. Biochemical & Biophysical Research Communications 87(3): 789-794

A method for the preparation of solvent-free phospholipid-impregnated filters is described. Polycarbonate filters of 13 mm diameter, 5 .mu.m thickness and 0.1 .mu.m pore size were employed, and 20-48 nmol phospholipid/filter was incorporated. Passive permeation of polar substances across the filter was determined by a flow-dialysis procedure. The presence of phospholipid led to a decrease in passive permeability by 96-99%. Due to their size and stability, and a partially preferential lipid orientation, these phospholipid-impregnated filters may serve as an alternative type of model membrane.

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Accession: 006173952

PMID: 454426

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