EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of stable erythrocyte target cells suitable for detection of the antibody response to the haptens azo benzene arsonate azo phenyltrimethyl ammonium and azo phenylphosphoryl choline by plaque forming cell assay


Journal of Immunological Methods 25(1): 89-96
Preparation of stable erythrocyte target cells suitable for detection of the antibody response to the haptens azo benzene arsonate azo phenyltrimethyl ammonium and azo phenylphosphoryl choline by plaque forming cell assay
The preparation of sheep red blood cells (SRBC) which were sensitized with the haptens azobenzenearsonate, phosphorylcholine and azophenyltrimethylammonium, and were suitable for use in a plaque-forming cell assay, was described. Each hapten was first coupled to a phenolic imidoester (methyl-p-hydroxybenzimidate) and the substituted imidoesters were employed to prepare target SRBC. Details were presented concerning synthesis and purification of the imidoesters and conditions for coupling to SRBC. The hapten-SRBC prepared by this method were very stable and offer significant advantages over other procedures for coupling these haptens to red blood cells.


Accession: 006173953

PMID: 370309



Related references

Preparation of stable erythrocyte target cells suitable for detection of the antibody response to the haptens azobenzenearsonate, azophenyltrimethylammonium and azophenylphosphorylcholine by plaque-forming cell assay. Journal of Immunological Methods 25(1): 89-96, 1979

Regulatory interactions among azo benzene arsonate specific helper and suppressor t cells in the in vitro antibody response to azo benzene arsonate proteins. Immunobiology 159(1-2): 174, 1981

Regulatory role of azo benzene arsonate specific helper and suppressor t cells in the in vitro antibody response to azo benzene arsonate proteins. Journal of Supramolecular Structure & Cellular Biochemistry (SUPPL 5): 78, 1981

Antigen driven and receptor driven regulatory mechanisms 8. suppression of idiotype negative p azo benzene arsonate specific t cells results from the interaction of an anti idiotypic second order t suppressor cell with a cross reactive idiotype positive p azo benzene arsonate primed t cell target. Journal of Experimental Medicine 153(6): 1415-1425, 1981

Hapten specific t cell response to azo benzene arsonate n acetyl l tyrosine in the lewis rat 3. effects of peptide spacer structure on eliciting azo benzene arsonate specific helper activity with tri nitro phenol hapten azo benzene arsonate peptide ficoll. Journal of Immunology 130(2): 586-589, 1983

Cells involved in the immune response. XXX. An evaluation of the different assays to demonstrate rabbit spleen plaque-forming cells. Enhancement of the assay by anti-target cell antibodies. Journal of Immunological Methods 46(1): 77-83, 1981

Cells involved in the immune response 30. an evaluation of different assays to demonstrate rabbit spleen plaque forming cells enhancement of the assay by anti target cell antibodies. Journal of Immunological Methods 46(1): 77-84, 1981

The detection of auto antibody forming cells part 1 an assay for plaque forming cells to the basic protein of myelin in guinea pigs. International Archives of Allergy & Applied Immunology 42(4): 627-640, 1972

Hapten specific t cell response to azo benzene arsonate n acetyl l tyrosine in the lewis rat 2. induction of helper activity demonstrated by tri nitro phenol haptenated azo benzene arsonate peptide ficoll. Journal of Immunology 130(2): 579-585, 1983

Preparation of di nitrofluoro benzene treated sheep erythrocytes for detection of anti di nitro phenyl plaque forming cells. Journal of Immunology 109(4): 754-760, 1972