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Preparation of stable erythrocyte target cells suitable for detection of the antibody response to the haptens azo benzene arsonate azo phenyltrimethyl ammonium and azo phenylphosphoryl choline by plaque forming cell assay


, : Preparation of stable erythrocyte target cells suitable for detection of the antibody response to the haptens azo benzene arsonate azo phenyltrimethyl ammonium and azo phenylphosphoryl choline by plaque forming cell assay. Journal of Immunological Methods 25(1): 89-96

The preparation of sheep red blood cells (SRBC) which were sensitized with the haptens azobenzenearsonate, phosphorylcholine and azophenyltrimethylammonium, and were suitable for use in a plaque-forming cell assay, was described. Each hapten was first coupled to a phenolic imidoester (methyl-p-hydroxybenzimidate) and the substituted imidoesters were employed to prepare target SRBC. Details were presented concerning synthesis and purification of the imidoesters and conditions for coupling to SRBC. The hapten-SRBC prepared by this method were very stable and offer significant advantages over other procedures for coupling these haptens to red blood cells.

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