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Preparation of sterile seawater through photodynamic action studies of vibrio vulnificus

, : Preparation of sterile seawater through photodynamic action studies of vibrio vulnificus. Microbios Letters 36(143-144): 153-156

Photodynamic action was tested as a means of managing Vibrio vulnificus under conditions of depuration. Experiments were conducted to determine the bactericidal effects of two sensitizers, rose bengal and eosin yellowish, on V. vulnificus, a representative marine bacterium that could cause human discomfort if present in shellfish which were consumed. Rose bengal was effective at micromolar concentrations, on the basis of comparing the most probable number (MPN) for test versus control cultures in seawater (30.permill.) medium. The effect showed selective toxicity in that rose bengal was significantly more effective than eosin yellowish, a trend which is consistent with the known ability of the two sensitizers to produce singlet oxygen.

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