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Preparation of sub viral particles from vaccinia virions irradiated with uv light


, : Preparation of sub viral particles from vaccinia virions irradiated with uv light. Journal of Virological Methods 1(4): 185-196

Nucleoprotein complexes, containing proteins covalently bound to DNA, were prepared from vaccinia virions irradiated with ultraviolet light. EM showed that these complexes had a spherical morphology with a densely staining central portion, apparently containing proteins cross-linked to DNA, from which loops or fibers of DNA or DNA complexed with protein emerged.

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