EurekaMag.com logo
+ Translate

Preparation of sub viral particles from vaccinia virions irradiated with uv light


, : Preparation of sub viral particles from vaccinia virions irradiated with uv light. Journal of Virological Methods 1(4): 185-196

Nucleoprotein complexes, containing proteins covalently bound to DNA, were prepared from vaccinia virions irradiated with ultraviolet light. EM showed that these complexes had a spherical morphology with a densely staining central portion, apparently containing proteins cross-linked to DNA, from which loops or fibers of DNA or DNA complexed with protein emerged.

(PDF 0-2 workdays service)

Accession: 006173975

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Soloski, M.; Holowczak, J.A., 1980: Preparation of subviral particles from vaccinia virions irradiated with ultraviolet light. Nucleoprotein complexes, containing proteins covalently bound to DNA, were prepared from vaccinia virions irradiated with ultraviolet light. In the electron microscope, these complexes were observed to have a spherical morphology with a densely st...

Soloski, M.J.; Cabrera, C.V.; Esteban, M.; Holowczak, J.A., 1979: The structure and organization of the vaccinia virus nucleoid 1. isolation and characterization of sub viral particles prepared by treating virions with guanidine hydro chloride nonidet p 40 and 2 mercapto ethanol. Subviral particles (Gu-subviral particles) containing DNA complexed with 20-25% of the protein found in complete virus particles could be isolated after treating purified vaccinia virions with 8 M guanidine-HCl, 1% Nonidet-P40 and 2-mercaptoethano...

Domber, E.; Holowczak, J.A., 1986: Vaccinia virus proteins on the plasma membranes of infected cells. Studies employing L cells infected with ultraviolet-irradiated vaccinia virions. Virology 152: 1-42

Domber, E.; Holowczak, J.A., 1986: Vaccinia virus proteins on the plasma membranes of infected cells. IV. Studies employing L cells infected with ultraviolet-irradiated vaccinia virions. As measured by in vitro, 51Cr-release assays, the expression on plasma membranes of two, immediate-early, vaccinia virus-specified cell-surface antigens, with mol wt of 25K-27K and 16K-17K, could be directly correlated with the susceptibility of t...

Domber, E.; Holowczak, J.A., 1986: Vaccinia virus proteins on the plasma membranes of infected cells iv. studies employing l cells infected with uv irradiated vaccinia virions. As measured by in vitro, 51Cr-release assays, the expression on plasma membranes of two, immediate-early, vaccinia virus-specified cell-surface antigens, with mol wt of 25K-27K and 16K-17K, could be directly correlated with the susceptibility of t...

Sharp, D.G.; McGuire, P.M., 1970: Spectrum of physical properties among the virions of a whole population of vaccinia virus particles. Populations of virions released from cultures of L cells infected with vaccinia virus are composed of particles which differ substantially from each other in sedimentation rate and buoyant density. Clumps of two and three virions sediment enough f...

Franke, C.A.; Hruby, D.E., 1987: Association of non-viral proteins with recombinant vaccinia virus virions. Evidence is presented which suggests that recombinant vaccinia virus particles (VV:CAT), containing the bacterial chloramphenicol acetyl transferase gene, are capable of encapsidating both the foreign protein which they encode (CAT) as well as cel...

Franke C.A.; Hruby D.E., 1987: Association of non viral proteins with recombinant vaccinia virus virions brief report. Evidence is presented which suggests that recombinant vaccinia virus particles (VV : CAT), containing the bacterial chloramphenicol acetyl transferase gene, are capable of encapsidating both the foreign protein which they encode (CAT) as well as c...

Lanzer, W.; Holowczak, J.A., 1975: Polyamines in vaccinia virions and polypeptides released from viral cores by acid extraction. Vaccinia virions propagated in the presence of [3H]ornithine were found to contain two labeled polyamines, spermine and spermidine. In complete virions the ratio of radioactively labeled spermine to spermidine was about 1:10, whereas in viral core...

Satheshkumar, P.S.; Weisberg, A.S.; Moss, B., 2013: Vaccinia virus A19 protein participates in the transformation of spherical immature particles to barrel-shaped infectious virions. The A19L open reading frame of vaccinia virus encodes a 9-kDa protein that is conserved in all sequenced chordopoxviruses, yet until now it has not been specifically characterized in any species. We appended an epitope tag after the start codon of...