EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of succinylated yeast protein composition and solubility


, : Preparation of succinylated yeast protein composition and solubility. Biotechnology and Bioengineering 20(9): 1329-1344

A method for the succinylation of yeast proteins during their isolation is described. Some factors affecting the extent of succinylation are described, e.g., heating of protein (80.degree. C for 5 min) prior to derivatization reduced maximum succinylation of available .epsilon.-amino groups from 88 to 54%. Succinylation of amino groups progressively increased as the concentration of succinic anhydride was increased and there was a concomitant decrease in nucleic acid content of the precipitated protein concentrate from 12.5 to 5.3 for 0 and 88% succinylation, respectively. Succinylation enhanced the solubility of the protein concentrates particularly below pH 7 and it decreased the isoelectric point from pH 4.5 to 4.0.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006173984

DOI: 10.1002/bit.260200903

PDF Full Text: Preparation of succinylated yeast protein composition and solubility


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Zeng, S-yuan.; Li, L.; Zhong, M-ling.; Jiang, W.; Wu, Y-yan.; Liu, Y., 2012: A randomized controlled trial of two chemotherapy regimens (paclitaxel liposome combined with platinum and paclitaxel combined with platinum) in concurrent chemoradiotherapy for cervical carcinoma. The aim of the study was to compare the efficacy, side effect and influence on the survival rate of two chemotherapy regimens, paclitaxel liposome combined with platinum and paclitaxel combined with platinum, in concurrent chemoradiotherapy for ce...

Yang, L.; Wong, C.Ming.; Chan, K.Pan.; Chau, P.Yuen.Kwan.; Ou, C.Quan.; Chan, K.Hung.; Peiris, J.S.Malik., 2009: Seasonal effects of influenza on mortality in a subtropical city. Influenza has been associated with a heavy burden of mortality. In tropical or subtropical regions where influenza viruses circulate in the community most of the year, it is possible that there are seasonal variations in the effects of influenza o...

Reefschläger, J.; Brwolff, D.; Bärwolff, D.; Langen, P.; Rosenthal, H.A., 1982: Efficiency and selectivity of (E)-5-(2-bromovinyl)-2'-deoxyuridine and some other 5-substituted 2'-deoxypyrimidine nucleosides as anti-herpes agents. A number of novel 5-substituted 2'deoxypyrimidine nucleosides exhibited antiviral activity against herpes simplex virus type 1 strain V3 (HSV-1-V3) when assayed under one-step conditions in primary human lung fibroblast j(PHLF) cell cultures,...

Thoms, H.; Wilson, H.M., 1937: Lateral Roentgenometry of the Pelvis-A Newly Modified Technic. Yale Journal of Biology and Medicine 9(4): 305-309

Lebkowski, J.; Schain, L.; Harvey, M.; Wysocki, M.; Biddle, W.; Moseley, A.; Okarma, T.B., 1993: Serum free culture of purified CD34+ cells yields large expansions of hematopoietic progenitors. Blood 82(10 SUPPL 1): 485A

Li, Q.; Zhao, X.; Liu, H.; Qu, F., 2014: Low pH capillary electrophoresis application to improve capillary electrophoresis-systematic evolution of ligands by exponential enrichment. In this work, a novel low pH CE-SELEX (LpH-CE-SELEX) as a CE-SELEX variant is proposed. Transferring (Trf), bovine serum albumin (BSA) and cytochrome c (Cyt c) as model protein are incubated with a FAM labeled ssDNA library, respectively. Incubati...

Kuester, R.K.; Sipes, I.G., 2007: Prediction of metabolic clearance of bisphenol A (4,4 '-dihydroxy-2,2-diphenylpropane) using cryopreserved human hepatocytes. This study investigated the kinetics of glucuronidation of bisphenol A (BPA; 4,4 '- dihydroxy- 2,2- diphenylpropane) in cryopreserved human hepatocytes (HCs). Incubation conditions were developed using Sprague- Dawley rat HCs. For determinat...

Lan, TC., 1991: A new trochid gastropod from north Australia. Bulletin of Malacology Republic of China, 16: 1-4

Lee, C.F.; Lin, S.J.; Lewis, C., 2002: Devising an integrated methodology for analyzing energy use and CO2 emissions from Taiwan's petrochemical industries. Input-output modeling and multiplier analysis are used to assess Taiwan's five petrochemical industries, based upon their economic contribution and potential impacts on energy consumption and CO2 emission. In addition, a consolidated index sy...

Romi, F., 2011: Thymoma in myasthenia gravis: from diagnosis to treatment. One half of cortical thymoma patients develop myasthenia gravis (MG), while 15% of MG patients have thymomas. MG is a neuromuscular junction disease caused in 85% of the cases by acetylcholine receptor (AChR) antibodies. Titin and ryanodine recept...