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Preparation of sulfur 35 labeled l methionine and l cystine by biosynthesis

, : Preparation of sulfur 35 labeled l methionine and l cystine by biosynthesis. Journal of Labelled Compounds and Radiopharmaceuticals 14(5): 727-734

A modified method for biosynthesis of L-methionine-35S and L-cystine-35S from baker's yeast (Saccharomyces cerevisiae) is described. The yeast was cultivated in a S-depleted medium containing carrier-free Na235SO4, under bubbling air for 24 h. Yeast proteins were hydrolyzed enzymatically into free amino acids, and L-methionine-35S and L-cystine-35S were isolated by ion-exchange column chromatography. Radioactive yields for L-methionine-35S and L-cystine-35S were 30% and 10%, respectively. The obtained products had high specific activity, 1-10 Ci/mmol, and radiochemical purity better than 95%.

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Related references

Skakun Todorovic M.B.; Albahari S.R., 1979: Preparation of sulfur 35 labeled l cysteine hydro chloride by reduction of sulfur 35 labeled l cystine. L-Cystine-35S obtained from baker's yeast was reduced with tin in HCl and radiochemically pure L-cysteine-35 S HCl was isolated by ion-exchange column chromatography. The obtained L-cysteine-35 S HCl was stable in aqueous solution, pH 2, for...

Torrens Y.; Michelot R.; Beaujouan J.C.; Glowinski J.; Bockaert J., 1982: In vivo biosynthesis of sulfur 35 labeled substance p from sulfur 35 labeled methionine in the rat striatum and its transport to the substantia nigra. Rats with a push-pull cannula implanted in the right striatum were used to study the biosynthesis of 35S-substance P (SP) from [35S]methionine and its transport to the ipsilateral substantia nigra. [35S]Methionine was delivered for 2, 3 or 5 h to...

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Anonymous, 1942: Radioactive sulfur studies I Synthesis of methionine* II Conversion of methionine sulfur to taurine sulfur in dogs and rats III Distribution of sulfur* in the proteins of animals fed sulfur* or methionine* IV Experiments in vitro with sulfur and hydrogen sulfide