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Preparation of sulfur 35 labeled l methionine and l cystine by biosynthesis


, : Preparation of sulfur 35 labeled l methionine and l cystine by biosynthesis. Journal of Labelled Compounds and Radiopharmaceuticals 14(5): 727-734

A modified method for biosynthesis of L-methionine-35S and L-cystine-35S from baker's yeast (Saccharomyces cerevisiae) is described. The yeast was cultivated in a S-depleted medium containing carrier-free Na235SO4, under bubbling air for 24 h. Yeast proteins were hydrolyzed enzymatically into free amino acids, and L-methionine-35S and L-cystine-35S were isolated by ion-exchange column chromatography. Radioactive yields for L-methionine-35S and L-cystine-35S were 30% and 10%, respectively. The obtained products had high specific activity, 1-10 Ci/mmol, and radiochemical purity better than 95%.

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