+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of sulfur 35 labeled rna and dna and their use for the assay of nucleolytic enzymes nucleosides and nucleotides 39

Chemical & Pharmaceutical Bulletin (Tokyo) 30(3): 1069-1072
Preparation of sulfur 35 labeled rna and dna and their use for the assay of nucleolytic enzymes nucleosides and nucleotides 39
Radioactive S was introduced into [yeast] RNA and [calf thymus] DNA molecules by chemical means. The thio group in 4-thiouracil residues, which were introduced into RNA and DNA by sulfhydrolysis, was exchanged with radioactive Na2S via a 4-thiocyanatouracil intermediate. Specific activity of the 35S-labeled RNA and DNA was around 105 cpm/ODU260 [optical density unit at 260 nm]. The labeled RNA and DNA were successfully applied as substrates for the assay of nucleolytic enzymes or RNase inhibitor. The sensitivity of the assay was 100 times better than that of the current method using non-labeled nucleic acids as the substrate.

Accession: 006173988

Related references

Preparation of 35S-labeled ribonucleic acid and deoxyribonucleic acid and their use for the assay of nucleolytic enzymes (nucleosides and nucleotides. XXXIX. Chemical & Pharmaceutical Bulletin 30(3): 1069-1072, 1982

The use of immobilized enzymes for the synthesis of nucleosides and nucleotides including the ones labeled with radioactive isotopes. Prikladnaia Biokhimiia i Mikrobiologiia 23(3): 309-316, 1987

Nucleosides and nucleotides. 135. DNA duplex and triplex formation and resistance to nucleolytic degradation of oligodeoxynucleotides containing syn-norspermidine at the 5-position of 2'-deoxyuridine. Bioconjugate Chemistry 6(1): 54-61, 1995

Nucleosides and Nucleotides: 135. DNA Duplex and Triplex Formation and Resistance to Nucleolytic Degradation of Oligodeoxynucleotides Containing syn-Norspermidine at the 5-Position of 2'-Deoxyuridine. Bioconjugate Chemistry 6(1): 54-61, 1995

Nucleosides and nucleotides 30. introduction of carbon substituents into pyrimidine and purine nucleosides by sulfur extrusion. Chemical and Pharmaceutical Bulletin (Tokyo) 28(1): 157-162, 1980

Preparation of dna labeled with high specific activity sulfur 35 labeled deoxy adenosine 5 alpha thio tri phosphate the use of sulfur 35 labeled nucleic acids as molecular hybridization probes. 1983

Preparation of labeled pyridine ribo nucleotides and ribo nucleosides. Mccormick, Donald B And Lemuel D Wright (Edited By) Methods in Enzymology Vol Xviii Vitamins And Coenzymes, Part B Xxi+857p Illus Academic Press: New York, N Y , U S A ; London, England 55-60, 1971

The preparation of sulfur 35 labeled homo cysteine thio lactone free of sulfur 35 labeled methionine. Journal of Biochemical & Biophysical Methods 7(1): 83-88, 1982

Unnatural nucleosides and nucleotides part 3 preparation of 2 carbon 14 and 4 carbon 14 labeled 5 alkyl uracils and 5 alkyl 2 deoxy uridines. Journal of Labelled Compounds and Radiopharmaceuticals 14(5): 713-726, 1978

Preparation of sulfur 35 labeled l cysteine hydro chloride by reduction of sulfur 35 labeled l cystine. Journal of Labelled Compounds & Radiopharmaceuticals 16(5): 711-716, 1979