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Preparation of technetium 99 di methyl sulfoxide renal complex in solution and its chemical and biological characterization


, : Preparation of technetium 99 di methyl sulfoxide renal complex in solution and its chemical and biological characterization. International Journal of Applied Radiation & Isotopes 33(4): 277-284

99Tc-DMS [dimethyl sulfoxide] solution was prepared by double isotope labeling, purified by molecular sieving through Sepharose 2B column, and checked by chromatographic and spectrophotometric methods, as well as by assessment of its biodistribution in rats. The preparations, obtained in a series of 9 experiments, showed high renal uptake (30-40% of injected dose/organ) and could be used for further investigations of Tc-DMS biochemical behavior in kidney tissue at subcellular level. Radioactive concentrations of purified 99Tc-DMS preparations were 37-74 kBq[kiloBequerels]/ml and absorption maxima at 412-425 nm (yellow complex). By application of 99mTc, 99Tc-DMS preparation in rats, good renal scans were obtained.

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