EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Primary culture of rat mammary epithelial cells part 2 cyto toxic effect and metabolism of 7 12 di methyl benz a anthracene and n nitroso n methyl urea



Primary culture of rat mammary epithelial cells part 2 cyto toxic effect and metabolism of 7 12 di methyl benz a anthracene and n nitroso n methyl urea



Journal of the National Cancer Institute 61(3): 773-778



To determine the cytotoxicity of 7,12-dimethylbenz[a]anthracene (DMBA) and N-nitroso-N-methylurea (NMU) on primary cultures of rat mammary cells, cultures were exposed to various concentrations of these carcinogens. Cytotoxicity was evident after exposure for 24 h to as little as 0.1 .mu.g DMBA/ml. Cytotoxicity was evident after exposure for 2 h with NMU at concentrations between 80 and 160 .mu.g/ml. Primary cultures of rat mammary cells were also examined for their ability to metabolize [3H]DMBA into water-soluble products. During a 48-h period, mammary cells could convert 2.2% [3H]DMBA to water-soluble metabolites. The cells retained (for at least 4 days) small quantities of [3H]DMBA that were insoluble in organic solvents.

(PDF emailed within 1 workday: $29.90)

Accession: 006182700

Download citation: RISBibTeXText



Related references

Effects of 7 12 di methyl benz a anthracene on rat mammary epithelial cells in culture. Federation Proceedings 38(3 PART 1): 1249, 1979

Effect of miscleron clofibrate on the carcinogenic effect of 7 12 di methyl benz a anthracene and nitroso methyl urea in female rats. Voprosy Onkologii (St Petersburg) 27(3): 64-67, 1981

Effect of various carcinogens 7 12 di methyl benz a anthracene 1 ethyl 1 nitroso urea on tritiated testosterone metabolism and dna synthesis in the rat prostate. Toxicology & Applied Pharmacology 37(1): 113, 1976

Metabolism of 7 12 di methyl benz a anthracene by rat mammary cells in culture. Proceedings of the American Association for Cancer Research 18: 210, 1977

Neoplastic transformation of rat mammary cells exposed to 7 12 di methyl benz a anthracene or n nitrosomethyl urea in cell culture. Proceedings of the National Academy of Sciences of the United States of America 75(8): 3836-3840, 1978

Primary culture of rat mammary epithelial cells. II. Cytotoxic effect and metabolism of 7,12-dimethylbenz[a]anthracene and N-nitroso-N-methylurea. Journal of the National Cancer Institute 61(3): 773-777, 1978

Hormone requirements for di methyl benz a anthracene induced alterations of rna synthesis in rat mammary epithelial cells in culture. In Vitro (Rockville) 15(3): 221-222, 1979

Bio transformation of 7 12 di methyl benz a anthracene on the genesis of 7 12 di methyl benz a anthracene trans di hydro diols and 7 12 di methyl benz a anthracene 7 methyl and 12 methyl hydroxylated metabolites. Biochemical and Biophysical Research Communications 93(3): 782-791, 1980

Comparative biologic activities of 7 12 di methyl benz a anthracene carcino 7 hydroxy methyl 12 methyl benz a anthracene carcino 7 12 di hydroxymethyl benz a anthracene carcino 4 methoxy 7 12 di methyl benz a anthracene carcino in the sprague dawley female rat. Cancer Research: 403-408, 1969

Influence of tamoxifen on ultrastructure and cell kinetics of 7 12 di methyl benz a anthracene induced mammary tumor cells in primary culture. IRCS (International Research Communications System) Medical Science Library Compendium 9(4): 281-282, 1981

Cyto photometric determination of the dna content in cells of experimental brain tumors part 2 9 10 di methyl 1 2 benz anthracene induced primary tumor of the rat cerebellum. Tsitologiya 18(8): 996-1002, 1976

Influence of tamoxifen and adriamycin on ultrastructure and surface topography of 7 12 di methyl benz a anthracene induced mammary tumor cells in primary culture. Journal of Cell Biology 91(2 PART 2): 109A, 1981

The effect of 9 10 di methyl 1 2 benz anthracene combined with anti pulmonary cyto toxic serum on rat lungs. Voprosy Eksperimental'noi Onkologii Respublikanskii Mezhvedomstvennyi Sbornik 4: TRANS 32-37, 1969

Effects of tamoxifen on cell sub populations of 7 12 di methyl benz a anthracene induced rat mammary tumor in primary culture. Anatomical Record 199(3): 17A, 1981