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Primary structure of an eco r i fragment of phage lambda imm 434 dna containing regions ci cro of phage 434 and cii o of phage lambda



Primary structure of an eco r i fragment of phage lambda imm 434 dna containing regions ci cro of phage 434 and cii o of phage lambda



Gene (Amsterdam) 6(3): 235-250



Digestion of phage .lambda.imm434 DNA with restriction endonuclease EcoRI yields 7 fragments. The shortest among them (1287 bp) contains the right part of the phage 434 immunity region and the phage DNA portion proximal to it. The complete primary structure of this fragment was determined using the chemical method of DNA sequencing. Hypothetical amino acid sequences of proteins coded by the cro gene of phage 434 and the cII gene of phage .lambda., and NH2-terminal amino acid sequences of the cI protein of phage 434 and the O protein of phage .lambda., were deduced solely on the basis of the DNA sequence. The fragment studied also contains the pR and probably prm promoters and the oR operator of phage 434. The sequence coding for them differs from the respective DNA sequence of phage .lambda.

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