+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Primary structure of chum salmon oncorhynchus keta prolactins occurrence of highly conserved regions

, : Primary structure of chum salmon oncorhynchus keta prolactins occurrence of highly conserved regions. Archives of Biochemistry & Biophysics 244(2): 528-541

The complete amino acid sequence of prolactin from the pituitaries of salmon (Oncorhynchus keta) has been determined. Salmon prolactin comprised two variants, I and II, which were separated by reverse-phase high-performance liquid chromatography. Each variant was reduced, S-carboxymethylated, and then cleaved with cyanogen bromide and enzymes. The resulting fragments were separated by reverse-phase high-performance liquid chromatography, as well as gel filtration, and subjected to sequence analysis by the dansyl-Edman method. Both variants contain 187 amino acid residues with two disulfide linkages at residues 46-160 and 177-187, lack a linkage in the N-terminal portion of mammalian prolactins, and differ from each other by the replacement of only four amino acid residues. Salmon prolactin (sPRL) showes 31% sequence identity with ovine prolactin. Moreover, four restricted regions, i.e., sPRL (3-21), (46-60), (68-83), and (160-178), encompass this significant conservatism between the teleost and the mammalian hormone, with identities of 47, 87, 62, and 68%, respectively. Such considerable identity between these distant phylogenic species strongly suggests that these regions may be responsible for the biological activity of prolactin.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006184842

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Ivkovic, Stjepan, 1948: Corvus frugilegus frugilegus L the nesting bird in Croatia. C. f. frugilegus has been known only as a common guest in winter. Between 1926 and 1948 the author found 5 colonies nesting in Croatia. All the colonies consisted of 10-100 pairs and the colonies had been nesting in the same sites for many years....

Vrinda, C.; Paradkar, S. N.; Jyotsna, N.; Sivaprasad, N., 2003: Immunoradiometric assay for human follicle stimulating hormone and luteinizing hormone using common avidin solid phase. This paper describes the use of avidin-biotin interaction as an affinity system, wherein avidin immobilized magnetizable particles (cellulose) are used as a common separation system in immunoradiometric assay (IRMA) for hormones of the human repro...

McGuire, Jimmy; Witt, Christopher; Altshuler, Douglas; Remsen, J. V., 2007: Phylogenetic systematics and biogeography of hummingbirds: Bayesian and maximum likelihood analyses of partitioned data and selection of an appropriate partitioning strategy. Hummingbirds are an important model system in avian biology, but to date the group has been the subject of remarkably few phylogenetic investigations. Here we present partitioned Bayesian and maximum likelihood phylogenetic analyses for 151 of app...

Evans Anthony M., 1995: Mineral deposit geology. Pages 29-43 1995

Worby, E., 1995: What does agrarian wage-labour signify? Cotton, commoditisation and social form in Gokwe, Zimbabwe. In the decade following Zimbabwe's Independence, the idea of a homogeneous, subsistence peasantry was soon dissolved by the 'discovery' of agrarian wage relations in the former African reserves. Simultaneously, residual collective l...

Phillips, R. E.; Quisenberry, V. L.; Zeleznik, J. M., 1995: Water and solute movement in an undisturbed, macroporous column: extraction pressure effects. Undisturbed soil columns are commonly taken to conduct water and solute flow experiments. The proper boundary condition to impose at the bottom boundary of a soil column is most difficult to define and control. The objective of this study was to e...

Tucker, H. A.; Paape, M. J.; Sinha, Y. N.; Pritchard, D. E.; Thatcher, W. W., 1967: Effects of nursing frequency on lactation, pituitary prolactin and adrenocorticotropic hormone content in rats. Lactating Sprague-Dawley rats, with the thoracic teats ligated on day 3, were allowed to nurse 6 young (0 1, (ii) 2 or (iii) 4 times daily or (iv) ad lib. from day 8 and the mammary nucleic acids were determined on day 16 and compared with non-nur...

Nielsen, Gunnlaugur P.; Stemmer Rachamimov, Anat O.; Ino, Yasushi; Moller, Michael B.; Rosenberg, Andrew E.; Louis, David N., 1999: Malignant transformation of neurofibromas in neurofibromatosis 1 is associated with CDKN2A/p16 inactivation. Patients with neurofibromatosis 1 (NF1) are predisposed to develop multiple neurofibromas (NFs) and are at risk for transformation of NFs to malignant peripheral nerve sheat tumors (MPNSTs). Little is known, however, about the biological events in...

Schwartz E.; Blumenfeld O.O.; Coltoff Schiller B.; Goldfischer S., 1984: Extracellular matrix microfibrils are composed of core protein coated with fibronectin. Journal of Cell Biology 99(4 PART 2): 76A

Song Sheng Rong; Lo Huann Jih, 1996: Lithofacies of volcanic rocks in the central Coastal Range, eastern Taiwan; implications to oceanic island arc evolution. International Geological Congress, Abstracts = Congres Geologique International, Resumes 30, VOL