Primary structure of rna polymerase from escherichia coli nucleotide sequence of the rpob gene fragment and corresponding amino terminal amino acid sequence of the beta subunit

Monastyrskaya, G.S.; Gubanov, V.V.; Gur'ev S.O.; Lipkin, V.M.; Sverdlov, E.D.

Bioorganicheskaya Khimiya 6(9): 1423-1426


ISSN/ISBN: 0360-4497
Accession: 006185033

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During the investigation of primary structures of E. coli DNA-dependent RNA polymerase .beta.-subunit and its structural gene rpoB, the nucleotide sequence of part of the EcoRI G fragment (645 base pairs), coding for the .beta.-subunit N-terminus, was established. The N-terminal .beta.-subunit amino acid sequence consisted of 188 amino acid residues. This is in accordance with sequences of isolated .beta.-subunit peptides and differs from the sequence, published by Gurevich et al. (1979).