Procedures for the automated analyses of proteoglycans and glycosaminoglycans
Ford, J.D.; Baker, J.R.
Analytical Biochemistry 84(2): 539-550
ISSN/ISBN: 0003-2697 PMID: 626397 DOI: 10.1016/0003-2697(78)90073-8
Methods for the automated analysis of hexose, uronic acid and protein using the Technicon AutoAnalyzer II were developed by modifying previously published procedures. A method of separating glucosamine and galactosamine, which is eminently suited to quantitating one in the presence of a large amount of the other, is reported. Procedures that can be recommended for determining the amino acid content and individual neutral sugars of proteoglycans or glycosaminoglycans are also described.