Production and isolation of purple pigment by alteromonas luteoviolacea
Mccarthy, S.A.; Sakata, T.; Kakimoto, D.; Johnson, R.M.
Nippon Suisan Gakkaishi 51(3): 479-484
Strains (11) of marine purple pigmented bacteria, isolated from coastal waters of Kinko Bay, Japan, were used. These isolates were identified as A. luteooviolacea based upon their bacteriological characteristics, primarily their low G + C base ratios (40.4-42.4 mol%). The 11 strains of A. luteoviolacea were divided into 2 major groups according to pigment production, HCN production and ability to grow at 37.degree. C. Of the 11 strains, 9 produced 2 purple pigments and 2 yellow pigments which were separated by Sephadex LH-20 column chromatography. Two strains, 9K-V9 and 9K-V10, produced only 2 purple pigments. The main fractions of the purple pigments were identified as violacein based upon absorption spectra and color change reactions. All strains produced purple pigment on solid media containing alanine or proline as a sole C source. Upon addition of tyrosine to yeast extract agar medium, 9K-V9 and 9K-V10 increased violacein production; all other strains produced brown pigment upon utilization of tyrosine. Violacein synthesis by 9K-V9 and 9K-V10 increased upon addition of tryptophan in concentration up to 200 mg/100 ml and decreased proportionally in the presence of higher concentrations of tryptophan.