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Production and partial purification of thermostable alpha amylase from streptomyces sp ha 40



Production and partial purification of thermostable alpha amylase from streptomyces sp ha 40



Korean Journal of Applied Microbiology & Bioengineering 15(3): 190-195



A strain of Streptomyces sp. (HA-40), isolated from soil, was able to produce a thermostable .alpha.-amylase. And the conditions for production and partial purification procedure were investigated. For production of the enzyme, starch and peptone was favorable as carbon and nitrogen source, respectively. And the enzyme production was promoted by the addition of Ca2+ and Na+, but depressed remarkably by Zn2+, Cu2+, Co2+, Hg2+, and Ag+. Optimal initial pH and temperature for the enzyme formation was pH 7.0 and 45.degree. C, respectively. The production of the enzyme reached at maximum in 2 days cultivation in the medium containing 1% starch, 0.2% peptone, 0.1% K2HPO4, 0.05% CaCl2, and 0.05% NaCl. The enzyme was partially purified about 42 folds from cultured filtrate by 80% methanol precipitation, DEAE-cellulose and Sephadex G-100 column chromatography with overall yield of 43%.

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Accession: 006190189

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