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Production and regeneration of protoplasts from pseudocercosporella herpotrichoides fron deighton



Production and regeneration of protoplasts from pseudocercosporella herpotrichoides fron deighton



Journal of Phytopathology (Berlin) 119(3): 193-205



This paper describes the development of a system for protoplast isolation from this pathogenic using the commercially available, hydrolytic enzyme preparations Rhozyme HP150, Driselase and Cellulase CP. An analysis of the active components of these enzymes believed to be involved in protoplast release has been made. Factors affecting protoplast release including mycelial age, enzyme concentration and choice of osmotic stabilizer have been optimised, and conditions for high frequency protoplast regeneration have been determined. The possible significance of the interaction of the several components of each enzyme is discussed. The developments described in this paper can now be exploited in genetic studies in this imperfect fungus.

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Accession: 006190316

Download citation: RISBibTeXText

DOI: 10.1111/j.1439-0434.1987.tb04389.x


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