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Production and tests of lyophilized standard preparation for diagnosis of mycoplasma pneumoniae in the indirect hem agglutination test



Production and tests of lyophilized standard preparation for diagnosis of mycoplasma pneumoniae in the indirect hem agglutination test



Voprosy Virusologii (1): 85-89



A method for the production of a reference lyophilized preparation for diagnosis of M. pneumoniae infections by indirect hemagglutination (IHA) test was developed. The method is based on conjugation using bi-diasotized benzidine of acrolein-treated sheep erythrocytes and ultrasonicated whole Mycoplasma antigen. After lyophilization the diagnostic preparation retained its standard properties for 1.5 yr (the observation period). The sensitivity of the preparation in the detection of antibody exceeded those of the CFT [complement fixation test] and the metabolic inhibition (MI) test by 16- to 64-fold and the specificity was as good as in these tests. The diagnostic value of the preparation in the IHA was 78.1%, in the CFT.sbd.64.7%. As compared with the CFT and the MI test, the IHA with Mycoplasma diagnostic preparation is simple and reproducible.

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Accession: 006190407

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