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Production by protoplast fusion of new strains of penicillium caseicolum for use in the dairy industry



Production by protoplast fusion of new strains of penicillium caseicolum for use in the dairy industry



Enzyme & Microbial Technology 8(1): 45-47



Protoplast fusion has been performed with strains of Penicillium caseicolum used in the dairy industry. Hybrids developed as diploids after a brief heterokaryon stage. The majority of the diploids had a level of sporulation similar to Penicillium caseicolum A, the less sporulating parental strain, while their growth rate was closer to the other, faster growing parental strain. But the variability between the sporulation rates of the different diploids was very weak. Prototrophic recombinants were selected after haploidization induced by benomyl. Unlike the diploids, the haploid recombinants exhibited a great variability in their morphological aspects (spore production, growth rate) and enzymatic properties (lipolysis, proteolysis). New strains with original combinations of the parental properties were then produced and selected.

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Accession: 006190464

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DOI: 10.1016/0141-0229(86)90010-4



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