Production of a chlorophyll deficient streptomycin resistant mutant of nicotiana tabacum for somatic hybridization studies

Toki, S.; Kameya, T.

Heredity 59(2): 223-226


ISSN/ISBN: 0018-067X
Accession: 006190683

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In order to produce a double mutant, sexual crosses between a streptomycin resistant mutant of Nicotiana tabacum (SR1) and a chlorophyll-deficient mutant of N. tabacum (Albino, A1) were carried out. From their offspring of this cross, a double mutant (SA) was selected and characterised with respect to streptomycin resistance and the ability to synthesize chlorophyll. Calli initiated from SA showed resistance to streptomycin and the chlorophyll content in SA leaves was lower than that of the normal green plants. Therefore, it is expected that the double mutant established here will be useful in studies of somatic hybridisation. Somatic hybrids between any other normal plants and the SA can be selected effectively by the appearance of green plants on a selective medium containing streptomycin.