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Production of a tea plant suspension cell culture



Production of a tea plant suspension cell culture



Fiziologiya Rastenii (Moscow) 26(2): 449-451



A suspension cell culture was successfully obtained from callus tissue cell aggregates of the tea-plant, Camellia sinensis, by deaggregation of cells with cellulase and pectinase with subsequent decantation of the suspension and its transfer to a fresh nutrient medium without enzymes. After 10 days the upper layer, consisting of small aggregates (up to 20-30 cells), and separate cells were removed and a calculation made of total cell numbers, number of living cells and phenol compound content. The remaining large aggregates in the lower layer were immersed again with fresh nutrient medium and continued to grow as the process was repeated. After three 10-day passages the number of cells and their viability significantly increased. The suspension culture of tea-plant stem cells thus obtained retained its capacity for synthesis of phenol compounds. This method may serve for the production of suspension cultures of other species of plants in those instances when the initial callus tissue exhibits a very dense consistency and is deaggregated with difficulty.

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Accession: 006190826

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