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Production of a thermostable lipase by humicola lanuginosa grown on sorbitol corn steep liquor medium

Production of a thermostable lipase by humicola lanuginosa grown on sorbitol corn steep liquor medium

Agricultural & Biological Chemistry 51(8): 2145-2152

For thermostable lipase production by Humicola lanuginosa No. 3, a simple optimized medium consisting of (%, w/v): sorbitol, 1.0; corn steep liquor, 1.0; NaCl, 0.5; CaCl2 .cntdot. 2H2O, 0.01; Silicone Km-70 (antifoamer), 0.2; and whale oil or castor oil as a lipase inducer, 0.3, was used. The yield of the lipase was about 80 .apprx. 120 U/ml after 25 hr aerobic cultivation at C when the pH was maintained at 7 to 8. The acetone powder preparation of the enzyme was most active at pH 7.0 and C. The enzyme retained 100% activity on incubation for 20 hr at C. The enzyme was able to hydrolyze almost all forms of natural fats tested (14 kinds), coconut oil being the most rapidly hydrolyzed.

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Accession: 006190828

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