EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Production of a thermostable lipase by humicola lanuginosa grown on sorbitol corn steep liquor medium



Production of a thermostable lipase by humicola lanuginosa grown on sorbitol corn steep liquor medium



Agricultural & Biological Chemistry 51(8): 2145-2152



For thermostable lipase production by Humicola lanuginosa No. 3, a simple optimized medium consisting of (%, w/v): sorbitol, 1.0; corn steep liquor, 1.0; NaCl, 0.5; CaCl2 .cntdot. 2H2O, 0.01; Silicone Km-70 (antifoamer), 0.2; and whale oil or castor oil as a lipase inducer, 0.3, was used. The yield of the lipase was about 80 .apprx. 120 U/ml after 25 hr aerobic cultivation at 45.degree. C when the pH was maintained at 7 to 8. The acetone powder preparation of the enzyme was most active at pH 7.0 and 45.degree. C. The enzyme retained 100% activity on incubation for 20 hr at 60.degree. C. The enzyme was able to hydrolyze almost all forms of natural fats tested (14 kinds), coconut oil being the most rapidly hydrolyzed.

(PDF 0-2 workdays service: $29.90)

Accession: 006190828

Download citation: RISBibTeXText



Related references

A comparative study of corn steep liquor, Bengal gram steep liquor and rice steep liquor on the production of penicillin by surface culture. Bull Torrey Bot Club 83(1): 30-34, 1956

Acidolysis reaction by a thermostable lipase from Humicola lanuginosa. Agricultural and Biological Chemistry 52: 23-5, 1988

Purification and some properties of a thermostable lipase from humicola lanuginosa no. 3. Agricultural and Biological Chemistry 51(1): 37-46, 1987

Hydrolysis of triglycerides by immobilized thermostable lipase from humicola lanuginosa. Agricultural & Biological Chemistry 52(1): 99-106, 1988

Biosurfactant production by Bacillus subtilis using corn steep liquor as culture medium. Frontiers in Microbiology 6: 59-59, 2015

Corn steep liquor as a nutrition adjunct for the production of Aspergillus niger lipase and hydrolysis of oils thereof. Journal of Agricultural and Food Chemistry 57(22): 10658-10663, 2010

Lipase production of a new thermophilic fungus humicola lanuginosa var catenulata. Journal of Fermentation Technology 64(5): 451-454, 1986

Production and characterization of cellulose by Acetobacter sp. V6 using a cost-effective molasses-corn steep liquor medium. Applied Biochemistry and Biotechnology 162(2): 486-497, 2010

A low-cost fermentation medium for thermophilic protease production by Streptomyces sp. 594 using feather meal and corn steep liquor. Current Microbiology 53(4): 335-339, 2006

Production of proteases by Bacillus sp. SMIA-2 grown on whey and corn steep liquor and compatibility of the enzyme with commercial detergents. Ciencia e Tecnologia de Alimentos 26(3): 582-588, 2006

Production of alpha-amylase in a corn steep liquor-soya bean meal medium by a strain of Bacillus stearothermophilus. Microbios 90(364): 155-162, 1997

High-level production of heterologous proteins using untreated cane molasses and corn steep liquor in Escherichia coli medium. Applied Microbiology and Biotechnology 87(2): 517-525, 2010

Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3. Brazilian Journal of Microbiology 42(4): 1384-1389, 2011

Production of glucose-fructose oxidoreductase and ethanol by Zymomonas mobilis ATCC 29191 in medium containing corn steep liquor as a source of vitamins. Applied microbiology and biotechnology 55(4): 442-445, 2001