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Production of alpha keto acids 2. immobilized whole cells of providencia sp pcm 1298 containing l amino acid oxidase ec 1.4.3.2



Production of alpha keto acids 2. immobilized whole cells of providencia sp pcm 1298 containing l amino acid oxidase ec 1.4.3.2



Enzyme and Microbial Technology 4(6): 409-413



A number of bacteria belonging to the genera Proteus, Providencia, Pseudomonas and Erwinia were tested for their capacity to oxidize L-amino acids to their corresponding .alpha.-keto acids. Members of the Proteus and the Providencia genera were active towards various L-amino acids. Immobilized cell preparations of Providencia sp. PCM 1298 form up to 80 mg .alpha.-keto-.gamma.-methiol butyric acid from L-methionine per g of gel preparation (containing 4% wt/wt cells) per day. The productivity was highly dependent on the size of the beads. O2 appeared to be the rate-limiting substrate, and O2 transfer rates of 3-4 .mu.mol cm-2 h-1 were calculated. The entrapment of activated charcoal to remove H2O2 formed during the oxidation extended the half-life of the immobilized biocatalyst considerably. A decrease in L-amino acid oxidase [L-amino acid: oxygen oxidoreductase (deaminating)] activity during operation could be compensated for by reinoculation of the alginate-entrapped cells in fresh growth medium, allowing use of these preparations of immobilized bacterial cells for > 1 mo.

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Accession: 006190939

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