Production of host specific toxins by helminthosporium victoriae and helminthosporium maydis in liquid shake culture
Van Etten, J.L.; Daly, J.M.
Phytopathology 70(8): 727-729
A method is described for growing a uniform suspension of H. victoriae hyphae in liquid shake culture or in a small fermentor. Such cultures produced host-specific toxin [oat seed germination bioassay] within a few days and the titers were equivalent to those produced in surface cultures grown for 3-4 wk. H. maydis host-specific toxin [corn leaf dark CO2 fixation bioassay] also was produced in liquid-shake culture. In addition to saving time, an advantage of shake or fermentation cultures was the rapid depletion of residual nutrients in the medium.