Production of sexual hybrids of nicotiana rustica x nicotiana tabacum and nicotiana rustica x nicotiana glutinosa via in vitro culture of fertilized ovules

Douglas, G.C.; Wetter, L.R.; Keller, W.A.; Setterfield, G.

Zeitschrift fuer Pflanzenzuechtung 90(2): 116-129

1983


Accession: 006193090

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Abstract
Interspecific crosses between N. rustica and N. tabacum or N. rustica and N. glutinosa are highly sterile because of abnormal ovule and embryo development. In vitro culture of excised N. rustica ovules after pollination by either of the other species allows significant numbers of hybrid embryos to develop into mature plants. Maximum numbers of normal seedlings were obtained following culture of placentae at 3 days post-pollination on B5 medium containing 2-4% sucrose. Yields of seedlings were improved by removing ovules from the placenta for culture. Higher yields of viable embryos were obtained from the N. rustica .times. N. tabacum cross than from the N. rustica .times. N. glutinosa cross. Hybrids from each cross were uniform in morphology and esterase isozyme composition and the majority were cytologically stable; they showed significant vegetative heterosis but flower characteristics were generally intermediate between those of the parents. All hybrids evaluated were self-sterile; N. rustica .times. N. tabacum hybrids yielded viable progeny when backcrossed to N. tabacum.