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Proline isomerization and the slow folding reactions of the alpha subunit of tryptophan synthase from Escherichia coli

Hurle, M.R.; Matthews, C.R.

Biochimica et Biophysica Acta 913(2): 179-184

1987

ISSN/ISBN: 0006-3002
PMID: 3297161
Accession: 006199535
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Previous studied on the refolding of the .alpha. subunit of tryptophan synthase from Escherichia coli assigned two slow refolding phases to rate-limiting isomerizations of two 'essential' proline residues, one in each of the two domains of the protein (Matthews, C.R., Crisanti, M.M., Manz, J.T. and Gepner, G.L. (1983) Biochemistry 22, 1445-1452). The double-jump experiment (Brandts, J.F., Halvorson, H.R. and Brennan, M. (1975) Biochemistry 14, 4953-4963) was used to further investigate this phenomenon. The reaction assigned to the carboxyl domain is consistent with the proline isomerization hypothesis. The amino domain process is more rapid than expected for proline isomerization and may reflect another type of slow folding reaction. The results permit a further refinement of the folding model for the .alpha. subunit and demonstrate the existence of a third unfolded species whose folding is not limited by either of these two reactions.

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Related References

Ziegelhoeffer A.; De Jong J.W.; Ferrari R.; Turi Nagy L. 1987: Proline isomerization and the slow folding reactions of the α subunit of tryptophan synthase from Escherichia coli Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology 913(2): 179-184
Stackhouse, T.M.; Onuffer, J.J.; Matthews, C.R.; Ahmed, S.A.; Miles, E.W. 1988: Folding of homologous proteins: conservation of the folding mechanism of the alpha subunit of tryptophan synthase from Escherichia coli, Salmonella typhimurium, and five interspecies hybrids Biochemistry 27(2): 824-832
Chrunyk, B.A.; Matthews, C.R. 1990: Role of diffusion in the folding of the alpha subunit of tryptophan synthase from Escherichia coli Biochemistry 29(8): 2149-2154
Chrunyk, B.A.; Matthews, C.R. 1990: The role of position 49 in the folding of the alpha subunit of tryptophan synthase from escherichia coli Biophysical Journal 57(2 Part 2): 436A
Chrunyk, B.A.; Tweedy, N.B.; Hurle, M.R.; Matthews, C.R. 1989: The effects of multiple replacements on the folding on the alpha subunit of tryptophan synthase from escherichia coli Journal of Cellular Biochemistry Suppl (13 Part A): 67
Nishio, K.; Morimoto, Y.; Ishizuka, M.; Ogasahara, K.; Tsukihara, T.; Yutani, K. 2005: Conformational changes in the alpha-subunit coupled to binding of the beta 2-subunit of tryptophan synthase from Escherichia coli: crystal structure of the tryptophan synthase alpha-subunit alone Biochemistry 44(4): 1184-1192
Gualfetti, P.J.; Bilsel, O.; Matthews, C.R. 1999: The progressive development of structure and stability during the equilibrium folding of the alpha subunit of tryptophan synthase from Escherichia coli Protein Science: a Publication of the Protein Society 8(8): 1623-1635
Crisanti, M.M.; Matthews, C.R. 1981: Characterization of the slow steps in the folding of the alpha subunit of tryptophan synthase Biochemistry 20(9): 2700-2706
Hurle, M.R.; Michelotti, G.A.; Crisanti, M.M.; Matthews, C.R. 1987: Characterization of a slow folding reaction for the alpha subunit of tryptophan synthase Proteins 2(1): 54-63
Stackhouse, T.M.; Onuffer, J.J.; Matthews, C.Robert 1988: Folding of homologous proteins: conservation of the folding mechanism of the a subunit of tryptophan synthase from Escherichia coli, Salmonella typhimurium, and five interspecies hybrids Biochemistry (American Chemical Society) 27: 4-32
Stackhouse, T.M.; Onuffer, J.J.; Matthews, C.R.; Ahmed, S.A.; Miles, E.W. 1988: Folding of hmologous proteins: conservation of the folding mechanism of the α subunit of tryptophan synthase from Escherichia coli, Salmonella typhimurium, and five interspecies hybrids Biochemistry (Easton) 27(2): 824-832
Chrunyk, B.A.; Matthews, C.Robert 1990: Role of diffusion in the folding of the a subunit of tryptophan synthase from Escherichia coli Biochemistry (American Chemical Society) 29: 49-54
Chrunyk, B.A.; Matthews, C.R. 1990: Role of diffusion in the folding of the α subunit of tryptophan synthase from Escherichia coli Biochemistry (Easton) 29(8): 2149-2154
Hodo, H.G.; Murphy, J.; Hardman, J.K.; Myers, R. 1977: Substrate interactions with the alpha-subunit of the Escherichia coli tryptophan synthase. a kinetic study of the wild-type alpha-subunit Archives of Biochemistry and Biophysics 181(2): 419-427
Nishio, K.; Morimoto, Y.; Ishizuka, M. 2005: Conformational Changes in the a-Subunit Coupled to Binding of the b2-Subunit of Tryptophan Synthase from Escherichia coli: Crystal Structure of the Tryptophan Synthase a-Subunit Alone Biochemistry (American Chemical Society) 44(4): 84-92