Section 7
Chapter 6,203

Properties of alkaline phosphatase in cucumber cucumis sativus cultivar seiriki no. 2 roots induced by calcium starvation

Yamaya, T.; Matsumoto, H.

Plant and Cell Physiology 22(7): 1355-1365


ISSN/ISBN: 0032-0781
Accession: 006202192

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Ca deficiency causes an increase in alkaline phosphatase activity in cucumber roots Matsumoto and Yamaya (1981). The activities of other hydrolases including acid phosphatase, nucleases and proteases were much less affected by the removal of Ca. Nucleoside di- and triphosphates and inorganic pyrophosphate were effectively hydrolyzed by the induced alkaline phosphatase, whereas nucleoside monophosphate-hydrolyzing activity was basically equal in the roots grown with either complete medium or a medium lacking Ca. The alkaline phosphatase in cucumber roots was found in fractions pelleting at 3,000 .times. g and in the 100,000 .times. g supernatant. The Ca-starved roots increased their alkaline phosphatase activity in both fractions. Four isozyme bands of the alkaline phosphatase in the soluble fraction were separated by polyacrylamide gel electrophoresis. One of the isozyme bands showed a prominent increase with the Ca deficiency, but not in the presence of cycloheximide.

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