EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Properties of amino terminal truncated yeast aspartyl transfer rna synthetase and structural characteristics of the cleaved domain


Properties of amino terminal truncated yeast aspartyl transfer rna synthetase and structural characteristics of the cleaved domain



European Journal of Biochemistry 174(1): 155-162



ISSN/ISBN: 0014-2956

Cytoplasmic aspartyl-tRNA synthetase from Saccharomyces cerevisiae is a dimer made up of identical subunits of Mr 64,000 as shown by biochemical and crystallographic analyses. Previous studies have emphasized the high sensitivity of the amino-terminal region (residues 1-32) to proteolytic enzymes. This work reports the results of limited tryptic or chymotryptic digestion of the purified enzyme which give rise to a truncated species that has lost the first 50-64 residues with full retention of both the activity and the dimeric structure. In contrast the larger tryptic fragment is distinguished from the whole enzyme by its weaker retention on heparin-substituted agarose gels. The cleaved N-terminal part presents peculiar structural features, such as a high content in lysine residues arranged in a palindromic fashion. The properties of the trypsin-modified enzyme and of the cleaved amino-terminal region are discussed in relation to the known structural characteristics of aspartyl-tRNA synthetase and of other eukaryotic aminoacyl-tRNA synthetases.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006202224

Download citation: RISBibTeXText

Related references

Properties of N-terminal truncated yeast aspartyl-tRNA synthetase and structural characteristics of the cleaved domain. European Journal of Biochemistry 174(1): 155-161, 1988

New photoactivatable structural and affinity probes of rna specific features and applications for mapping of spermine binding sites in yeast aspartyl transfer rna and interaction of this transfer rna with yeast aspartyl transfer rna synthetase. Nucleic Acids Research 18(1): 89-96, 1990

Yeast aspartyl transfer rna tertiary structure in solution and areas of interaction of the transfer rna with aspartyl transfer rna synthetase a comparative study of the yeast phenylalanine system by phosphate alkylation experiments with ethylnitrosourea. Journal of Molecular Biology 184(3): 455-472, 1985

Cloning and primary structure of the gene coding for yeast cytoplasmic aspartyl transfer rna synthetase mapping of the 5' and 3' termini of aspartyl transfer rna synthetase messenger rna. Biological Chemistry Hoppe-Seyler 366(9): 854, 1985

Crystallographic studies on the aspartyl transfer rna synthetase aspartyl transfer rna system from yeast the crystalline aminoacyl transfer rna synthetase. Journal of Molecular Biology 138(1): 129-136, 1980

Yeast aspartyl transfer rna aspartyl transfer rna synthetase recognition the crystal structure of a class ii system. Journal of Biomolecular Structure and Dynamics 8(6): A186, 1991

Structural and functional role of the amino-terminal region of porcine cytosolic aspartate aminotransferase. Catalytic and structural properties of enzyme derivatives truncated on the amino-terminal side. Journal of Biological Chemistry 266(7): 4187-4193, 1991

Structural and functional role of the amino-terminal region of porcine cytosolic aspartate aminotransferase : catalytic and structural properties of enzyme derivatives truncated on the amino-terminal side. The Journal of Biological Chemistry 266(7): 4187-4193, 1991

Yeast aspartyl transfer rna aspartyl transfer rna synthetase the crystal structure of a class ii system. Journal of Cellular Biochemistry Suppl (15 Part D): 152, 1991

Isolation purification and study of some properties of lysyl transfer rna synthetase phenyl alanyl transfer rna synthetase and aspartyl transfer rna synthetase from the fungus verticillium dahliae. Khimiya Prirodnykh Soedinenii 1974(2): 229-233, 1974

Crystallography of the complex aspartyl transfer rna aspartyl transfer rna synthetase from yeast. Bianconi, A And A Congiu Castellano (Ed ) Springer Series in Biophysics, Vol 2 Biophysics And Synchrotron Radiation Xii+333p Springer-Verlag: Berlin, West Germany; New York, New York, Usa Illus 10-18, 1987

Large scale purification and structural properties of yeast aspartyl-tRNA synthetase. Biochemical and Biophysical Research Communications 117(1): 259-267, 1983

Asparaginyl transfer rna synthetase from escherichia coli has significant sequence homologies with yeast aspartyl transfer rna synthetase. Gene 84(2): 481-486, 1989

2 domain structure of alanyl transfer rna synthetase from bombyx mori isolation of the amino terminal catalytic domain. Journal of Biochemistry 96(6): 1875-1882, 1984

Purification and properties of aspartyl transfer rna synthetase and valyl transfer rna synthetase from muscles of normal and long fasting rabbits. Doklady Akademii Nauk Ukrainskoi SSR Seriya B Geologicheskie Khimicheskie i Biologicheskie Nauki 9: 63-67, 1983