EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Proton extrusion by leaf discs of vicia faba l. light and ion stimulated proton release



Proton extrusion by leaf discs of vicia faba l. light and ion stimulated proton release



Biologia Plantarum (Prague) 30(2): 124-130



Previous electrophysiological and tracer kinetic studies indicated that the uptake of neutral amino acids took place by means of the proton cotransport mechanism in the leaf tissue of broad bean plants. The present investigations were designed to characterize the origin of the driving force for this process, and the proton pumping activity of leaf cells of Vicia. This activity is known to be revealed when peeled broad been leaf discs, floated on a bathing solution in the light or in darkness acidify the medium. White light caused the strongest acidification. The presence of K+ and Na+ in the external solution increased the H+ secretion significantly, whereas addition of Ca++ caused only an insignificant enhancement of proton extrusion. The inhibitors of photosynthetic electron transport DCMU [3-(3,4-dichlorophenyl)-1,1-dimethylurea] (50 .mu.M) and nitrofen (50 .mu.M) eliminated the light-enhanced H+ release indicating the dependence on photosynthesis. The involvement of a proton pump was evidenced by the effects of the uncoupler CCCP [carbonyl cyanide-m-chlorophenylhydrazone] the SH reagent HgCl2 and the ATPase inhibitor orthovanadate. The experimental results support the conclusion that H+ extrusion by Vicia leaf cells is an active electrogenic process requiring metabolic energy. In the light this energy requirement is supplied via photosynthetic electron transport.

(PDF 0-2 workdays service: $29.90)

Accession: 006215506

Download citation: RISBibTeXText



Related references

Proton extrusion by leaf discs of Vicia faba L.: light- and ion-stimulated H+ release. Biologia Plantarum 30(2): 124-130, 1988

Proton extrusion by leaf discs of vicia faba l. influence of inhibitors and phytohormones on proton extrusion. Biologia Plantarum (Prague) 30(4): 252-259, 1988

Proton extrusion by leaf discs of Vicia faba L.: Influence of inhibitors and phytohormones on H+ extrusion. Biologia Plantarum 30(4): 252-259, 1988

Blue light-dependent proton extrusion by guard-cell protoplasts of Vicia faba. Nature 319(6051): 324-326, 1985

Effect of osmotic pressure on proton extrusion, and on the uptake of K+ (86Rb) and of sugars and amino acids by leaf tissues of Vicia faba L. Comptes Rendus de l' Academie des Sciences, III Sciences de la Vie 299(10): 435-440, 1984

Effect of osmotic pressure on proton extrusion, and on the uptake of K+(86Rb) and of some organic nutrients (sugars, amino acids) by leaf tissues of Vicia faba L. Comptes rendus des seances de l'Academie des Sciences Serie III Sciences de la Vie, 299(10): 435-440, 1984

Effect of osmotic pressure on proton extrusion and on the uptake of potassium rubidium 86 and of some organic nutrients sugars amino acids by leaf tissues of vicia faba. Comptes Rendus de l'Academie des Sciences Serie III Sciences de la Vie 299(10): 435-440, 1984

Release of Sucrose from Vicia faba L. Leaf Discs. Plant Physiology 71(2): 333-340, 1983

Proton sugar co transport in leaf tissues of vicia faba. Plant Physiology (Rockville) 63(5 SUPPL): 44, 1979

Effects of light and inhibitors on glutamate metabolism in vicia faba leaf discs. Plant Physiology (Rockville) 61(4 SUPPL): 53, 1978

Proton Fluxes Associated with Sugar Uptake in Vicia faba Leaf Tissues. Plant Physiology 68(3): 706-711, 1981

Blue light-dependent proton extrusion by guard-cell protoplasts of Vivia faba. Nature 29, 319(6051): 324-326, 1986

Proton fluxes associated with sugar uptake in vicia faba cultivar aguadulce leaf tissues. Plant Physiology (Rockville) 68(3): 706-711, 1981

The effect of red and far-red light on proton secretion from mesophyll-cell protoplasts of Vicia faba L. Planta 173(1): 42-45, 1988