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Proton nmr of intercalators and guanylyl 3' 5' cytidylyl 3' 5' adenosine a potential mutagenicity probe



Proton nmr of intercalators and guanylyl 3' 5' cytidylyl 3' 5' adenosine a potential mutagenicity probe



Chemico Biological Interactions 50(1): 97-110



Variable temperature 1H-NMR was used to study the interaction of the RNA trimer, GpCpA, with the intercalators ethidium bromide and the acridine derivatives; proflavin, 9-aminoacridine, acridine orange, acridine yellow and acriflavin. The complexes formed were studied at nucleic acid to drug ratios of 1:1 and 5:1, the latter being useful in defining the effects of structural variation in the acridine series and in determining the site of intercalation. All the intercalators greatly stabilized the oligonucleotide duplex, the average melting temperature (Tm) increasing by up to 30.degree. C. Significant changes in individual Tm and chemical shifts were observed for all the GpCpA protons. 9-Aminoacridine and acriflavin did not stabilize the GpCpA duplex as substantially as the other acridine derivatives. This intercalator:GpCpA system and its associated NMR-derived Tm may be a useful physical probe for potential mutagens.

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