EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Proton nmr study of the 2 calcium binding sites of porcine intestinal calcium binding protein



Proton nmr study of the 2 calcium binding sites of porcine intestinal calcium binding protein



Journal of Biological Chemistry 260(14): 8342-8347



1H NMR was employed to study the Ca-binding properties of the NH2-and COOH-terminal Ca-binding sites of the porcine intestinal Ca-binding protein. The protein was titrated with Ca in the presence of the chelator EDTA to determine the association constants of the protein for Ca relative to the known association constant of EDTA for Ca. The resulting data were comapred with various models for the binding of Ca to 2 sites on the protein. Models were considered for which the 2 sites in the apoprotein have either intrinsically equal or unequal affinities for calcium. For each of these 2 cases, positive cooperativity, no cooperativity, and negative cooperativity were considered. The data fit best for the case of random binding to 2 independent sites with equivalent association constants of 1.0 .+-. 0.1 .times. 107 M-1. The case of ordered binding to 2 sites with intrinsically different affinities, with concomitant positive affinity between the 2 sites so that the effective association constants were made equal, could not be mathematically excluded when only 1 protein NMR resonance is considered but can be shown to be implausible when the whole spectrum is considered.

(PDF 0-2 workdays service: $29.90)

Accession: 006216023

Download citation: RISBibTeXText



Related references

1H nuclear magnetic resonance study of the two calcium-binding sites of porcine intestinal calcium-binding protein. Journal of Biological Chemistry 260(14): 8342-8347, 1985

Structural differences in the two calcium binding sites of the porcine intestinal calcium binding protein: a multinuclear NMR study. Biochemistry 24(15): 3870-3876, 1985

Structural differences in the 2 calcium binding sites of the porcine intestinal calcium binding protein a multinuclear nmr study. Biochemistry 24(15): 3870-3876, 1985

Proton nuclear magnetic resonance studies of the interaction of the lanthanide ions ytterbium and lutetium with apo- and calcium-saturated porcine intestinal calcium binding protein. Biochemistry (American Chemical Society) 24: 32-8, 1985

Proton nmr studies of porcine intestinal calcium binding protein. Biochemistry 22(11): 2649-2654, 1983

Proton nmr studies of the interaction of the lanthanide ions ytterbium and lutetium with apo saturated and calcium saturated porcine intestinal calcium binding protein. Biochemistry 24(9): 2332-2338, 1985

Porcine intestinal calcium-binding protein. Molecular properties and the effect of binding calcium ions. Journal of Biological Chemistry 249(1): 199-204, 1974

Proton nmr studies of ytterbium substituted porcine intestinal calcium binding protein. Canadian Journal of Biochemistry & Cell Biology 63(9): 992-997, 1985

Quantitative analysis of calcium binding to porcine intestinal calcium-binding protein. Journal of Biochemistry 93(2): 487-493, 1983

Proton nuclear magnetic resonance studies of porcine intestinal calcium binding protein. Biochemistry 22(11): 2649-2654, 1983

Observations on the binding of lanthanides and calcium to vitamin D-dependent chick intestinal calcium-binding protein. Implications regarding calcium-binding protein function. Journal of Biological Chemistry 262(14): 6539-6545, 1987

Low affinity calcium binding sites verus zinc binding sites in histidine rich calcium binding protein of skeletal muscle sarcoplasmic reticulum. Biochemical & Biophysical Research Communications 186(2): 659-667, 1992

Radio immunoassay studies of intestinal calcium binding protein 4 in the pig part 1 identification of intestinal calcium binding protein in blood and response to a low calcium diet. Canadian Journal of Physiology & Pharmacology 53(6): 1129-1134, 1975