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Protoplast fusion between saccharomyces cerevisiae and candida lipolytica conditions for protoplast fusion



Protoplast fusion between saccharomyces cerevisiae and candida lipolytica conditions for protoplast fusion



Korean Journal of Applied Microbiology & Bioengineering 14(1): 17-22



Two different auxotrophic protoplasts of Saccharomyces cerevisiae and Candida lipolytica were fused with the aid of polyethylene glycol (PEG) and calcium ions. These auxotrophic strains were induced by treatment of N-methyl-N'-nitro-N-nitrosoguanidine (NTG), and they were used for protoplast fusion. The fusion efficiency was much higher by incubating protoplasts in PEG (MW 4,000) solution (pH 8.0) for 10 min and by using potassium chloride for osmotic stabilizer. The intergeneric fusion frequency was 104 to 105, equivalent to one-tenth of intraspecific fusion frequency.

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Accession: 006216451

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