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Protoplast fusion in the yeast schwanniomyces alluvius



Protoplast fusion in the yeast schwanniomyces alluvius



Molecular & General Genetics 186(1): 95-100



This 1st application of the technique of protoplast fusion to Schwanniomyces suggests that it is possible to overcome the genetic isolation of this genus imposed by its inability to undergo conventional intraspecific mating. The stability, increased ploidy, and cell volumes of such fusion hybrids over the parental strains indicate the possibility of construction of polyploid strains suitable for use in industry. Nuclear fusion (karyogamy) apparently occurs in intraspecific hybrids as evidenced by isolation of recombinants after mitotic segregation of parental auxotrophic genetic markers. Intergeneric hybrids formed from S. alluvius and Saccharomyces spp. were unstable and spontaneously segregated into original auxotrophic parent cultures. Genetic diversity between these genera may be too great to allow stable co-existence of the 2 genomes within a single nucleus. Nuclear fusion in such cases could not be confirmed.

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Accession: 006216464

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DOI: 10.1007/bf00422918



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