+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Purification and characterization of 3 hydroxy 3 methylglutaryl coenzyme a reductase from potato tubers



Purification and characterization of 3 hydroxy 3 methylglutaryl coenzyme a reductase from potato tubers



Journal of Biochemistry (Tokyo) 100(4): 967-974



3-Hydroxy-3-methylglutaryl coenzyme A reductase (NADPH) was solubilized by trypsin digestion from sliced potato tuber microsomes, and purified to apparent homogeneity in the absence of detergent with a recovery of 1.8%. The enzyme had a specific activity of 7,910 nmol of mevalonate formed per min per mg of protein. On molecular-sieving high-performance liquid chromatography, the activity was coincident with the single protein peak corresponding to a molecular weight of approximately 110 kDa. On SDS-polyacrylamide gel electrophoresis, the purified enzyme showed only one protein staining band corresponding to a molecular weight of approximately 55 kDa. The apparent Km value for S-HMG-CoA was 6.4 .mu.M and that for NADPH was 25 .mu.M.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006225546

Download citation: RISBibTeXText


Related references

Purification and characterization of 3-hydroxy-3-methylglutaryl CoA reductase from potato tubers. Journal of Biochemistry 100(4): 967-974, 1986

Purification and Characterization of 3-Hydroxy-3-Methylglutaryl CoA Reductase from Potato Tubers. The Journal of Biochemistry 100(4): 967-974, 1986

Induction of 3 hydroxy 3 methylglutaryl coenzyme a reductase in potato tubers after slicing fungal infection or chemical treatment and some properties of the enzyme. Plant and Cell Physiology 26(5): 873-880, 1985

Purification and characterization of 3-hydroxy-3-methylglutaryl coenzyme A reductase from chicken liver. Febs Letters 80(1): 123-129, 1977

Splice donor site mutations in the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene cause a deficiency of the endoplasmic reticulum 3-hydroxy-3-methylglutaryl coenzyme A reductase protein in UT2 cells. Journal of Lipid Research 39(11): 2182-2191, 1998

Modulation of regulatory oxysterol formation and low density lipoprotein suppression of 3 hydroxy 3 methylglutaryl coenzyme a reductase activity by ketoconazole a role for cytochrome p 450 in the regulation of 3 hydroxy 3 methylglutaryl coenzyme a reductase in rat intestinal epithelial cells. Journal of Biological Chemistry 261(18): 8348-8356, 1986

Mevalonate utilization in Pseudomonas sp. M. Purification and characterization of an inducible 3-hydroxy-3-methylglutaryl coenzyme A reductase. Journal of Biological Chemistry 260(16): 9393-9398, 1985

Regulation of cytosolic acetoacetyl coenzyme A thiolase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and mevalonate kinase by low density lipoprotein and by 25-hydroxycholesterol in Chinese hamster ovary cells. Journal of Biological Chemistry 255(16): 7787-7795, 1980

3-Hydroxy-3-methylglutaryl-coenzyme A reductase from Haloferax volcanii: purification, characterization, and expression in Escherichia coli. Journal of Bacteriology 178(1): 19-23, 1996

Purification and characterization of fermodulin an iron dependent inhibitor protein of 3 hydroxy 3 methylglutaryl coenzyme a reductase. Archives of Biochemistry and Biophysics 239(2): 342-351, 1985

Partial purification and characterization of 3 hydroxy 3 methylglutaryl coenzyme a reductase from the leaves of guayule parthenium argentatum. Phytochemistry 25(11): 2471-2474, 1986

Purification, characterization, and cloning of a eubacterial 3-hydroxy-3-methylglutaryl coenzyme A reductase, a key enzyme involved in biosynthesis of terpenoids. Journal of Bacteriology 181(4): 1256-1263, 1999

Detergent-solubilization, purification, and characterization of membrane-bound 3-hydroxy-3-methylglutaryl-coenzyme A reductase from radish seedlings. European Journal of Biochemistry 154(1): 103-111, 1986

Identification of elements critical for phosphorylation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by adenosine monophosphate-activated protein kinase: protein engineering of the naturally nonphosphorylatable 3-hydroxy-3-methylglutaryl coenzyme A reductase from Pseudomonas mevalonii. Biochemistry 36(5): 1157-1162, 1997

Calcium ionophore treatment impairs the sterol mediated suppression of 3 hydroxy 3 methylglutaryl coenzyme a reductase 3 hydroxy 3 methylglutaryl coenzyme a synthase and farnesyl diphosphate synthetase. Journal of Biological Chemistry 267(4): 2831-2836, 1992