+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Purification and characterization of a protein from escherichia coli which complexes with super helical and single stranded dna species



Purification and characterization of a protein from escherichia coli which complexes with super helical and single stranded dna species



Journal of Biochemistry 92(4): 1059-1068



From the cells of an E. coli K-12 strain, a 22,000-dalton protein which has an affinity for the superhelical DNA molecule was purified to apparent homogeneity by monitoring the DNA-binding activity using the filter binding assay. In the sedimentation analysis of the DNA-protein complex, the protein has an affinity for the superhelical or single-stranded DNA molecule but not for open circular DNA or for linear DNA molecules. The amino acid composition of the protein resembled those of the other prokaryotic histone-like proteins and also to eukaryotic histones H2A and H2B. The protein precipitated upon heating, which is in contrast to the heat-stable feature of the other histone-like proteins. DNA and RNA syntheses in vitro were not affected by the presence of the protein. This protein may play a role in maintaining the bacterial nucleoid structure.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006225990

Download citation: RISBibTeXText


Related references

Purification and characterization of a protein from Escherichia coli which forms complexes with superhelical and single-stranded DNAs. Journal of Biochemistry 92(4): 1059-1068, 1982

Purification and Characterization of a Protein from Escherichia coli which Forms Complexes with Superhelical and Single-Stranded Dnas. The Journal of Biochemistry 92(4): 1059-1068, 1982

Purified escherichia coli reca protein catalyzes homoeologous pairing of super helical dna and single stranded fragments. Proceedings of the National Academy of Sciences of the United States of America 76(4): 1638-1642, 1979

Continuous association of Escherichia coli single-stranded DNA binding protein with stable complexes of recA protein and single-stranded DNA. Biochemistry 25(7): 1482-1494, 1986

Purification and characterization of the Escherichia coli RecO protein. Renaturation of complementary single-stranded DNA molecules catalyzed by the RecO protein. Journal of Molecular Biology 236(1): 124-138, 1994

Effects of escherichia coli ssb protein on the single stranded dna dependent atpase activity of escherichia coli reca protein evidence that ssb protein facilitates the binding of reca protein to regions of secondary structure within single stranded dna. Journal of Molecular Biology 193(1): 97-114, 1987

Interaction between dna and escherichia coli dna topo isomerase i formation of complexes between the protein and super helical and nonsuperhelical duplex dna. Journal of Biological Chemistry 254(21): 11082-11088, 1979

Escherichia coli single-strand binding protein forms multiple, distinct complexes with single-stranded DNA. Biochemistry 25(24): 7799-7802, 1986

Stabilization of recA protein-ssDNA complexes by the single-stranded DNA binding protein of Escherichia coli. Biochemistry 29(3): 837-843, 1990

Stable complexes between escherichia coli protein omega and single or double stranded dna. Federation Proceedings 35(7): 684, 1976

Escherichia coli ssb protein forms multiple distinct complexes with single stranded dna. Biophysical Journal 51(2 Part 2): 149A, 1987

Triplet state properties of tryptophan residues in complexes of mutated Escherichia coli single-stranded DNA binding proteins with single-stranded polynucleotides. Biophysical Journal 55(5): 927-936, 1989

Two binding modes in Escherichia coli single strand binding protein-single stranded DNA complexes. Modulation by NaCl concentration. Journal of Biological Chemistry 260(6): 3594-3603, 1985

Uptake of homologous single stranded fragments by super helical dna part 2 characterization of the reaction. Journal of Molecular Biology 116(4): 783-804, 1977

Energy transfer in complexes of E. coli single-stranded DNA-binding protein with single-stranded poly-(2-thiouridylic acid). Febs Letters 261(2): 389-391, 1990