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Purification of leghemoglobin messenger rna from alfalfa medicago sativa root nodules and in vitro translation



Purification of leghemoglobin messenger rna from alfalfa medicago sativa root nodules and in vitro translation



Korean Biochemical Journal 18(1): 71-76



The 5 components of leghemoglobin from alfalfa root nodules were purified by DEAE-cellulose (DE52) and Sephadex G-100 chromatography and antiserum against the purified alfalfa leghemoglobin was prepared. RNA was extracted from alfalfa root nodules and poly(A)-mRNA was purified by oligo(dT)-cellulose affinity chromatography. The leghemoglobin messages were observed to be 23% of the poly(A)-mRNA. The poly(A)-mRNA was fractionated by sucrose density gradient centrifugation and the mRNA had 9, 18 and 28S value populations with 58, 21 and 36% of the leghemoglobin messages.

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