Section 7
Chapter 6,234

Purification of some tricarboxylic acid cycle enzymes from beef heart using affinity elution chromatography

Davies, J.R.; Scopes, R.K.

Analytical Biochemistry 114(1): 19-27


ISSN/ISBN: 0003-2697
PMID: 6269463
DOI: 10.1016/0003-2697(81)90444-9
Accession: 006233646

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Applying the principles of affinity elution chromatography, 8 different mitochondrial enzymes of, or associated with, the tricarboxylic acid cycle were purified from beef heart. Conditions were found for adsorbing each enzyme to CM-cellulose, and eluting each with its substrate(s). After a gel filtration step, and in some cases use of an affinity adsorbent, most of these preparations had specific activities comparable to the highest previously reported.

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