Purification of some tricarboxylic acid cycle enzymes from beef heart using affinity elution chromatography
Davies, J.R.; Scopes, R.K.
Analytical Biochemistry 114(1): 19-27
1981
ISSN/ISBN: 0003-2697 PMID: 6269463 DOI: 10.1016/0003-2697(81)90444-9
Accession: 006233646
Applying the principles of affinity elution chromatography, 8 different mitochondrial enzymes of, or associated with, the tricarboxylic acid cycle were purified from beef heart. Conditions were found for adsorbing each enzyme to CM-cellulose, and eluting each with its substrate(s). After a gel filtration step, and in some cases use of an affinity adsorbent, most of these preparations had specific activities comparable to the highest previously reported.