+ Translate

Quantitation of anthracyclines in human hematopoietic cell subpopulations by flow cytometry correlated with high pressure liquid chromatography

, : Quantitation of anthracyclines in human hematopoietic cell subpopulations by flow cytometry correlated with high pressure liquid chromatography. Cytometry 6(2): 143-150

The major white cell subpopulations present in bone marrow and peripheral blood can be discriminated by forward and perpendicular light scatter two-parameter flow cytometry (FCM). Fluorescent properties of anthracycline antibiotics allow measurement of the concentration of these cytotoxic drugs in hematopoietic cells by FCM as a third parameter. Analysis of scatter-gated fluorescence histograms provides quantitative information about the cellular concentration of at least four cell categories in human blood and bone marrow cells. A good correlation was found between the mean cellular fluorescence measured by FCM and the overall cellular concentration of adriamycin, daunomycin, and their main metabolites determined with high-pressure liquid chromatography (HPLC). In incubation experiments with human hematopoietic tissues, the final concentration of various anthracyclines in subpopulations of white cells appeared to be dependent on cell density, incubation time, temperature, and type of compound and its concentration. FCM analysis is a rapid, sensitive, and quantitative method for measurement of cellular anthracycline concentrations in subpopulations and therefore provides an useful new tool in monitoring chemotherapy.

Accession: 006239315

PMID: 3856510

DOI: 10.1002/cyto.990060210

Download PDF Full Text: Quantitation of anthracyclines in human hematopoietic cell subpopulations by flow cytometry correlated with high pressure liquid chromatography

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Speth P.; Linssen P.; Boezeman J.; Wessels H.; Haanen C., 1984: Pharmacokinetics of anthracyclines in plasma and hematopoietic cells determined by flow cytometry and high performance liquid chromatography. British Journal of Haematology 58(1): 191-192

Schaefer, P.C.; Goldsmith, R.S., 1978: Quantitation of 25-hydroxycholecalciferol in human serum by high-pressure liquid chromatography. An HPLC method for assaying 25(OH)D3 in extracts of human serum is described. The method involves extraction of serum with methanol-dichloromethane and chromatography on a column of Sephadex LH-20 prior to HPLC with quantitation by UV absorbance....

Mewis, C.; Kühlkamp, V.; Lindena, I.; Risler, T.; Ress, K., 1996: Quantitation of pentisomide and its metabolite by high-pressure liquid chromatography in human serum. A rapid, inexpensive, and accurate high-performance liquid chromatographic method for the simultaneous determination of pentisomide and its major mono-N-dealkylated metabolite has been developed. After a simple and inexpensive solvent extraction p...

Huisman, T.H.; Gardiner, M.B.; Wilson, J.B., 1981: Experiences with the quantitation of human hemoglobin types by high pressure liquid chromatography. Progress in Clinical and Biological Research 60: 69-82

Piccolo D.E.; Kupfer D., 1979: Quantitation of prostaglandins in human semen by reverse phase high pressure liquid chromatography. Hawk, G L (Ed ) Chromatographic Science Series, Vol 12 Biological/Biomedical Applications Of Liquid Chromatography 2; Symposium, Boston, Mass , Usa, Oct 5-6, 1978 Xiii+504p Marcel Dekker, Inc : New York, N Y , Usa; Basel, Switzerland Illus P425-436

Knaus, E.E.; Coutts, R.T.; Kazakoff, C.W., 1976: The separation, identification, and quantitation of cannabinoids and their tau=butyldimethylsilyl, trimethylsilylacetate, and diethylphosphate derivatives using high-pressure liquid chromatography, gas-liquid chromatography, and mass spectrometry. Methods for the separation, identification, and quantitation, of cannabinoids present in hashish have been developed. These methods include high-pressure liquid chromatographic and gas-liquid chromatographic separation of cannabinol, delta 9-tetra...

Bye, A.; Brown, M.E., 1977: An analytical approach to the quantitation of known drugs in human biological samples by high pressure liquid chromatography. Although many HPLC, [high pressure liquid chromatography] methods are available only a fraction of these are applicable to the analysis of known drugs in human biological fluids [blood, urine, etc.]. A favored approach for the quantitative assay o...

Bender J.G.; Walker D.E.; Unverzagt K.L.; Lee W.; Vanepps D.E.; Stewart C.; Smith D., 1990: Quantitation of subpopulations of cd34 positive cells present in normal human bone marrow using multiparameter flow cytometry. Cytometry (SUPPL 4): 28

Orntoft, T.F.; Petersen, S.E.; Wolf, H., 1988: Dual-parameter flow cytometry of transitional cell carcinomas. Quantitation of DNA content and binding of carbohydrate ligands in cellular subpopulations. Quantitative DNA measurements and estimates of blood group-related carbohydrate antigen expression have been used as predictive parameters in transitional cell carcinomas (Ca). To obtain an accurate quantitative characterization of cellular subpop...

Van Lear, G.E.; Chiccarelli, F.S.; Bonenfant, P.A.; Barr, A., 1978: Quantitation of the anti-inflammatory agent fenbufen and its metabolites in human serum and urine using high-pressure liquid chromatography. Specific procedures are described for the determination of fenbufen and its metabolites in serum and urine using high-pressure liquid chromatography. Serum or urine extracts were chromatographed on a bounded reversed-phase partitioning column. The...