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Quantitation of genetic differences between trypanosoma brucei gambiense trypanosoma brucei rhodesiense and trypanosoma brucei brucei by restriction enzyme analysis of kinetoplast dna



Quantitation of genetic differences between trypanosoma brucei gambiense trypanosoma brucei rhodesiense and trypanosoma brucei brucei by restriction enzyme analysis of kinetoplast dna



Molecular & Biochemical Parasitology 3(2): 117-131



Recognition sites (44) were compared for 12 restriction endonucleases on the 20 kbase pair maxi-circle to kinetoplast DNA from 9 T. brucei stocks, 4 are infective to man (2 gambiense and 2 rhodesiense variants). In addition to 5 polymorphic sites, this DNA differs in the size of a 5 kbase pair region which is cleaved only by 1 of the restriction enzymes tested and which varies in size over 1.5 kbase pairs. The maxi-circle sequences of these stocks are evidently very similar, the maximal calculated difference between any 2 being 3%. A relatively large difference was found between a rhodesiense stock from Uganda and 1 from Zambia, confirming the distinction between northern and southern East African rhodesiense stocks found by analysis of enzyme polymorphisms. The gambiense variants could not be identified by unique restriction site polymorphisms, but contained the smallest maxi-circle found in T. brucei. T. brucei stocks infective and not infective to man apparently are so closely related as to preclude their differentiation by analysis of kinetoplast DNA. This analysis is useful in providing quantitative information about relatedness of stocks.

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