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Quantitative localization of the phytoalexin glyceollin i in relation to fungal hyphae in soybean glycine max cultivar harosoy roots infected with phytophthora megasperma f sp glycinea



Quantitative localization of the phytoalexin glyceollin i in relation to fungal hyphae in soybean glycine max cultivar harosoy roots infected with phytophthora megasperma f sp glycinea



Plant Physiology 77(3): 591-601



A radioimmunoassay specific for glyceollin I was used to quantitate this phytoalexin in roots of soybean after infection with zoospores of either race 1 (incompatible) or race 3 (compatible) of P. megasperma Drechs. f.sp. glycinea Kuan and Erwin. The sensitivity of the radioimmunoassay and an immunofluorescent stain for hyphae permitted quantitation of phytoalexin and localization of the fungus in alternate serial cryotome sections from the same root. The incompatible interaction was characterized by extensive fungal colonization of the root cortex which was limited to the immediate vicinity of the inoculation site. Glyceollin I was 1st detected in extracts of whole roots 2 h after infection, and phytoalexin content rose rapidly thereafter. Significant concentrations of glyceollin I were present at the infection site in cross-sections (42 .mu.m thick) of such roots by 5 h, and exceeded 0.6 .mu.mol/ml (EC90 [effective concentration] in vitro for glyceollin I) by 8 h after infection. Longitudinal sectioning (14 .mu.m thick) showed that glyceollin I accumulated particularly in the epidermal cell layers, but also was present in the root cortex at inhibitory concentrations No hyphae were observed in advance of detectable levels of the phytoalexin and, in most roots, glyceollin I concentrations dropped sharply at the leading edge of the infection. The compatbile interaction was characterized by extensive unchecked fungal colonization of the root stele, with lesser growth in the rest of the root. Only small amounts of glyceollin I were detected in whole root extracts during the first 14 h after infection. Measurable amounts of glyceollin I were detected only in occasional cross-sections of such roots 11 and 14 h after infection. The phytoalexin was present at inhibitory concentrations in the epidermal cell layers, but the inhibitory zone did not extend appreciably into the cortex. The accumulation of glyceollin I apparently is an important early response of soybean roots to infection by P. megasperma, but may not be solely responsible for inhibition of fungal growth in the resistant response.

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Accession: 006244527

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